L11 distinguishing drivers from passengers

Question 1

how can we use bioinformatics to identify drivers? what software can we use for each?

Answer 1

identify genes mutated more frequently than background mutation rate- mutsigcv, genome music

identifying genes that have functional impact- SIFT prediction tool, oncodriveFM

identifying mutations that cluster together in regions of high impact- oncodriveCLUST

Question 2

what is a disadvantage of using oncodriveFM?

Answer 2

loses sensitivity because in-silico prediction tools of function don’t always get it right
because they are computerised.