L11

Question 1

Examining gene expression at the transcript level: in situ hybridization

Answer 1

Whole developing organism, add labelled RNA probe, add enzyme-conjugated AB, add enzyme-substrate, view spatial distribution of mRNA with light microscope

Question 2

Immunostaining

Answer 2

whole developing organism, add antibody against protein, add flurophore-conjucated ab against ab, visualize with fluorescence microscope