L1 DNA structure + electrophoresis Flashcards
1
Q
Prokaryotic, Virus Eukaryotic common
A
Nucleic acids (DNA and RNA)
2
Q
DNA structure basic concepts
A
Base > Sugar backbone > Phosphate group to link the sugars
3
Q
Complementary base pairing
A
4 Bases in DNA
A+T
C+G
H-bonds
4
Q
Gel electrophoresis
A
Agarose gel:
300-20,000 bp analysis
Polyacrylamide gel:
5-500 bp analysis
5
Q
Pulse field electrophoresis
A
For large DNA molecules.
Uses magnetic field in all direction to increase the distance traveled by individual molecules
30kbp - 10Mbp separation.
6
Q
Electrophoretic Mobility shift assay (EMSA)
A
Overview: Binding of a protein to a DNA slows its migration during electrophoresis.
This allows analysis of binding properties of proteins
7
Q
Gel electrophoresis standard curve
A
Migration is not proportional to size, but proportional to the Log (size)
