L. 3 Drugs and Diagnostic Enzymes

Question 1

Competetive Enzyme Inhibitor Drugs

Answer 1

-Competes with substrate by non-covalently binding at active site
-Unchanged Vmax -Higher Km (lower affinity)
Statins: Lowers cholesterol
Ibuprofen: NSAID inhibits cycloxygenase -> No prostoglandin
Captopril: Lowers BP

Question 2

Active Site-Directed (Transitiion-State) Drugs

Answer 2

-Bind to active site with covalent linkage
Organophosphates: Neurotoxin, inhibits acetylcholinesterase -> no ACh breakdown
Cyclooxygenase: NSAID, inhibits cycloxygenase -> No prostoglandin

Question 3

Enzyme-Activated (Suicide) Drugs

Answer 3

-Reacts with enzyme and gets altered, making it bind very tightly to enzyme
Penicillin: Antibiotic, inhibits cross-linking in bacterial wall
Allopurinol: Gout Tx, inhibits uric acid formation

Question 4

AST

Answer 4

• Liver, skeletal, muscle, liver, brain

- Liver disease

Question 5

ALT

Answer 5

• Liver

- Liver disease

Question 6

Amylase

Answer 6

• Pancrease, salivary gland

- Acute pancreatitis, Biliary obstruction

Question 7

CK

Answer 7

• Skeletal/cardiac muscle, brain

- Muscular dystrophy, MI

Question 8

GGT

Answer 8

• Liver

- Hepatits, cirrhosis

Question 9

LDH

Answer 9

• heart, liver erythtocytes

- Lymphoma, hepatitis

Question 10

Lipase

Answer 10

• Pancreas

- Acute pancreatitis, Biliary obstruction

Question 11

Alkaline Phosphatase

Answer 11

• Osteoblast

- Bone disease/tumor

Question 12

Acid Phosphatase

Answer 12

• Prostate

- Prostate cancer