Kinetics of Balanced Growth Flashcards
The rate of cell growth varies depending on the _______
growth phase
The different phases of growth are more readily distinguished when the _______ of viable cell concentration is plotted against time
logarithm
To find the relationship or graph of the cell concentration vs time, ______ plot is used
semi-log
What are the six phases of cell growth
Lag phase, Acceleration, Growth phase, Decline phase, stationary phase, Death phase
During the __________ immediately after inoculation of the culture, the rate of growth is
essentially zero
lag phase
Lag phase refers to a period of time when the change of cell number is
zero
Cells use the ________ to adapt to their new environment.
lag phase
In this lag phase, new enzymes or structural components may be synthesized but the
concentration of cells ____________
does not increase
The length of this lag period depends on many factors such as
the type and age of the microorganisms, the size of the inoculum, and culture conditions
Growth starts for the inoculated cell culture
Acceleration phase
At acceleration phase, the cell number starts to ______, and the division rate increases to reach a __________
increase, maximum
During this period, growth achieves its maximum rate
Growth phase
If growth is ___________ nutrient exhaustion or build-up of inhibitory products. (which is the most often case), the growth phase appears as a
straight line on a semi-log plot
exponential
During decline phase, growth slows down due to____________
nutrient exhaustion or build-up of inhibitory products.
During this period, cell growth ceases
Stationary phase
No further cell growth can be observed.
Stationary phase
The transition between the exponential phase and the stationary phase involves a period
of _____________
unbalanced growth
cells in the stationary phase have a chemical composition _________ from that of cells in the exponential phase.
different
At this phase, cells lose viability or are destroyed
Death phase
During death phase, the cells are destroyed by _______
lysis
Death occurs either because of
depletion of the cellular reserves of energy, or the accumulation of toxic products.
cell growth is considered to be a
first-order autocatalytic reaction
In a closed system where growth is the only process affecting cell concentration, the rate of growth 𝑟𝑁 is _______ to the rate of change of cell concentration
equal
Cell growth rates are often expressed in terms of the _________
doubling time, 𝒕d
time required for the cell population to double
doubling time, 𝒕d
doubling time is a valid representation of the growth rate only when ____________
μ is constant.
