Kinetic Quantitation & Inhibition of Catechol Oxidase

Question 1

enzymes

Answer 1

are molecules that act catalysts to speed up chemical reactions - most enzymes are proteins

Question 2

active site of an enzyme

Answer 2

has a specific tertiary shape and binds the substrate with high specificity

Question 3

product

Answer 3

molecules are released from active site

Question 4

an enzyme assay

Answer 4

is a laboratory method for measuring enzyme activity

Question 5

our assay in experiment……

Answer 5

Enzyme + Substrate = Product
Catechol oxidase + catechol = benzoquinone (brown product)

Question 6

Factors that influence enzyme activity

Answer 6

1.pH
2. co-factors & co-enzymes
3. inhibitors
4. Temperature

Question 7

reaction rate depends……

Answer 7

on concentration of enzymes and substrate/reactant

Question 8

Vmax

Answer 8

maximum velocity of the reaction

Question 9

Vo =

Answer 9

= Vmax [S] / Km + [S]

Question 10

Km =

Answer 10

= Michaelis Menten constant, [S] when Vo is 1/2 the Vmax

Question 11

Vo

Answer 11

initial velocity

Question 12

Km

Answer 12

is a measure of the affinity of the enzymes for the substrate

Question 13

an enzymes inhibitor

Answer 13

is a molecule that binds to an enzyme and decreases its activity

Question 14

sodium metabisulfite

Answer 14

reducing agent which denatures the enzymes, can inhibit Catechol oxidase