Key Points Document Flashcards
Describe the light dependent reaction
Chlorophyll absorbs light energy and excites its electrons
Electrons removed and chlorophyll oxidised via photoionisation
Electrons move along Electron Transport Chain, releasing energy via REDOX reactions
Energy released used to create an electrochemical gradient
H+ ions diffuse through ATP synthase
Providing the energy to join ADP and Pi into ATP via photophosphorylation
Photolysis of water produces 2 protons, 2 electrons and 0.5 oxygen
NADP reduced by electrons
Describe the light independent reaction
Carbon dioxide combines with RuBP Producing 2 GP GP reduced to Triose Phosphate Using reduced NADP Using energy from ATP Triose Phosphate converted to glucose
ATP is produced in the light dependant reaction
Suggest why this is not plants only source of ATP
Plants don’t photosynthesise in the dark
Not all parts of a plant photosynthesise
Plants require more ATP than is produced in the light dependant reaction
ATP is used in active transport
Effect of introducing a herbicide/inhibitor on the electron transport chain
Reduced transfer of protons across the thylakoid membrane
Reduced electrochemical gradient across thylakoid membrane
So less ATP
So less reduced NADP
So light independant reaction slows
Less GP reduced to Triose Phosphate
Describe what happens during photoionisation in the light dependant reaction
Chlorophyll absorb light which excites electrons
Electrons are lost and move down the electron transport chain
When producing a chromatogram explain why the origin is marked using a pencil rather than pen
Ink and leaf pigments would mix
Origin would be in different place with pen
With pencil the origin is still visible and in same position
While making a chromatogram describe the method used to separate the pigments after the solution of pigment has been applied to origin
Level of solvent below origin line
Remove before solvent reaches the top
Suggest and explain the advantage of having different colour pigments in leaves
Absorb different/more wavelengths of light for photosynthesis
Describe how oxidation tales place in glycolysis and the krebs cycle
Removal of hydrogen (dehydrogenation)
By enzymes (dehydrogenase)
H accepted by NAD (forms reduced NAD)
In Krebs cycle, H accepted by FAD (forms reduced FAD)
Water is a waste product of aerobic respiration
Describe how water is formed at the end of aerobic respiration
Oxygen is the final electron acceptor
Combines with protons and electrons to form water
Explain why converting pyruvate to lactate allows the continued production of ATP by anaerobic respiration
Regenerates NAD/NADH oxidised
So glycolysis can continue
Explain how the amount of ATP is increased by reactions occurring inside a mitichondria
Oxidation/removal of electrons and hydrogen
From pyruvate
Acetyl CoA the 6 carbon compound
Substrate level phosphorylation produces ATP in krebs cycle
Producing NADH and FADH
In the matrix of the mitochondria
Electrons move into the ETC and pass along carriers
Releasing energy
Protons move into innermembrsne space
ADP and Pi using ATP synthase for ATP
Why is a log scale used to record the numbers of cells/bacteria
Large range/difference in numbers
Reference to exponential increase
Give two reasons why a respirometer is left for 10 minutes when first placed in the bath
Equilibrium reached
Allow for expansion/pressure change in apparatus
Allow respiration rate of x to stabilise
The mitochondria in muscles contain many cristae
Why is this an advantage
Larger surface area for electron carrier system/oxidative phosphorylation
More room for attachment of ATP synthase
Provide ATP so energy for muscle contraction
Describe the role of coenzyme and carrier protein in ATP synthesis
(Describe the events of oxidative phosphorylation)
NAD/FAD reduced by hydrogen
H+ ions transferred from coenzyme to coenzyme in the ETC on cristae of inner membrane
Energy released through series of redox reactions
Energy used to pump protons into innermembrane space
H+ diffuses through ATP synthase to produce ATP from ADP and Pi
Biomass
Mass of carbon (organic compounds)
Dry mass of tissue per given area
What do you do to remove all water from a tissue sample
Regularly weigh and heat
Until mass is constant
GPP
Gross Primary Productivity
Chemical energy stored in plant biomass in a given area or volume (rate of photosynthesis)
NPP
Net Primary Production
Chemical energy stored in plant biomass after respiratory losses to the environment have been taken into account (NPP=GPP-R)
Available for new plant growth, reproduction or for other trophic levels in the ecosystem (like herbivors or saprobionats)