Kaplan FL 1 Flashcards BIOCHEM 1
what do competitive inhibitors do?
they INCREASE Km
Vmax satys the same
How does competitive inhibitor look on Lineweaver Burke plot?
Lineweaver Burk
x intercept closer to 0 (bigger Km)
same y intercept (same Vmax)
How does competitive inhibitor look on Michealis Menton plot?
V max is the same
Km increases which means more substrate is needed to reach Vmax
Km is 1/2 Vmax
What does Lineweaver Burke plot look like
What does the Michealis Menten plot look like?
cooperative enzymes
Certain enzymes do not show the normal hyperbola when graphed on a Michaelis–Menten plot (v vs. [S]), but rather show sigmoidal (S-shaped) kinetics owing to cooperativity among substrate binding sites
Subunits and enzymes may exist in one of two states: a low-affinity tense state (T) or a high-affinity relaxed state (R). Binding of the substrate encourages the transition of other subunits from the T state to the R state, which increases the likelihood of substrate binding by these other subunits. Conversely, loss of substrate can encourage the transition from the R state to the T state, and promote dissociation of substrate from the remaining subunits.,
example of cooperative binding enzyme in glycolysis
Enzymes showing cooperative kinetics are often regulatory enzymes in pathways, like phosphofructokinase-1 in glycolysis. Cooperative enzymes are also subject to activation and inhibition, both competitively and through allosteric sites.
The cooperative binding of hemoglobin, which acts as a transport protein rather than an enzyme, results in a characteristic sigmoidal binding curve that is an MCAT favorite.
Which peptide gives same results as H-LAPAT-MPAA?
H-LAPAC-MPAA
H-LAPAP-MPAA
H-LAPAM-MPAA
H-LAPAS-MPAA
look for a amino acid change most similar to T
T is threonine which has a hydroxy group.
S is serine which also has a hydroxy group
if a molecule is used to prevent undesirable reactions then what kinds of amino acids is it preventing
it would protect against reactive groups like amino acids with R groups and reactive functionalities like NH2 OH SH and COOH. Arginine is an example of a mino acid with a positively charged gruanidino group with amino groups
