KA1: laboratory techniques for biologists: detecting proteins using antibodies Flashcards

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1
Q

what are immunoassay techniques used to detect and identify?

A

specific proteins

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2
Q

what do immunnoassay techniques use?

A

stocks of antibodies with the same specificity, known as monoclonal antibodies

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3
Q

what is an antibody specific to the protein antigen linked to?

A

a chemical “label”

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4
Q

what is this “label”?

A

a reporter enzyme producing a colour change c

chemiluminescence, fluorescence and other reporters can be used

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5
Q

in some cases, what does the assay use to detect the presence of antibodies?

A

a specific antigen

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6
Q

what is western blotting?

A

technique used after SDS–PAGE electrophoresis

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7
Q

describe the use of western blotting

A

The separated proteins from the gel are transferred (blotted) onto a solid medium

The proteins can be identified using specific antibodies that have reporter enzymes attached

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