Jose Jimenez MCQs Flashcards
When checking the resulting products of a PCR by gel electrophoresis…
Shorter fragments will migrate faster
Different restriction enzymes …
recognise specific sequences and produce ends that are occasionally compatible
DNA ligases are responsible for:
the formation of phosphodiester bonds between DNA fragments
What steps are involved in cloning of bacteria?
DNA amplification
DNA digestion
DNA ligation
Electroporation
A plasmid that is transfected into animal cells and does not contain an origin of replication but carries a selection marker may lead to…..
A stable integration in the chromosome and spread in the population in the presence of the selection marker
Northern blot allows:
To determine the size of a transcript
To detect the presence of a transcript
What technique would be used to identify the cells expressing a particular gene in the development of the endoderm of Drosophila melanogaster
In situ hybridisation
Protein fusions allow studying
both dynamics of expression and protein localisation thanks to the presence of a reporter
The most probable outcome of the cross of two heterozygous individuals each carrying a dominant mutation is:
75% of progeny will have mutant phenotype
25% of progeny will have a mutant phenotype
The most probably outcome of two heterozygous individuals each carrying a recessive mutation is:
25% of progeny will have mutant genotype
25% will have mutant phenotype
Mutations are always
changes in the DNA sequence
The minimal requirement in order to be affected with Huntington’s disease is:
One parent must be a carrier
Which part of RNA polymerase subunits is not part of the processive core and gets released after initiation
sigma
Which part of the sigma factors will not bind to -10 and -35 regions
Sigma 54
Two component regulatory systems involve
A histidine kinase that phosphorylates a regulator when an inducer is present
