JC_ENH 1 Flashcards
predominant protein
albumin
CF: Albumin
10
CF: Bilirubin
17.1
CF: BUN
0.357
CF: Na
1
CF: K
1
CF: Cl
1
CF: Chole
0.026
CF: Crea
88.4
CF: Glucose
0.0555
CF: Thyroxine
12.9
CF: T4
12.9
CF: Total Protein
10
CF: Trigly
0.0113
CF: Uric acid
0.0595
Conversion:
Conv. to SI
conv. x CF
Conversion:
SI to conv.
SI / CF
Convert 126 mg/dL glucose to SI unit
126 x 0.0555 = 7 mmol/L
chemical substance subject of chemical analysis
Analyte
compound or mixture added to a system to cause a chemical reaction or test if a reaction occurs
Reagent
Term reagent is often used in place of __________.
reactant
T/F:
A reagent may not necessarily be consumed in a reaction like a reactant.
TRUE
anything that speeds up the rate of a chemical reaction but is not being produced or consumed in the reaction
Catalyst
process of testing and adjusting analyzer’s readout to establish correlation between measured and actual conc.
Calibration
a material of known conc.
Standard/Calibrator
sample that is chemically and physically similar to unknown spx and is tested in exactly the same manner
Control
set of introduction for methods used in the laboratory
SOP
type of rgt water with maximum water purity
Type I
rgt water used for ultramicrochem analysis
Type I
rgt water used for nano/subnano analysis
Type I
rgt water used for analysis of trace metals
Type I
rgt water used for analysis of drugs
Type I
rgt water used for analysis of hormones
Type I
rgt water used for tissue culture
Type I
rgt water used in most lab tests and routine tests
Type II
rgt water used for CBC
Type II
rgt water used for gram staining
Type II
rgt water used for rgt prep and QC
Type II
rgt water used for most qualitative tests
Type III
rgt water used in histology
Type III
rgt water known as tap water
Type III
rgt water used for U/A and F/A
Type III
expressed as the “% solution”
Concentration
Concentration formula
% / 100 = x / vol/wt
mol/L
Molarity
Eq/L
Normality
mol = ?
gmw
Eq = ?
gmw/v
valence = # of ___
Hydrogen
Dilution formula
vol of spx / (vol of spx + vol of diluent)
Corrected dilution formula
value obtained for diluted spx x reciprocal of dilution
centrifuge that is vertical at rest, horizontal in motion
swinging bucket/horizontal rotor
swinging bucket
air friction:
speed:
sediment:
swinging bucket
air friction: inc
speed: dec
sediment: poor
centri faster than swinging bucket due to less air friction which allows more rapid sedimentation
fixed/angle
head rotor of fixed centri
25-40 deg
centri with high speed and is refrigerated to reduce heat
Ultracentrifuge
ultracentri rpm
100,000 rpm
ultracentri is mainly used for?
lipoprotein analysis, to remove trubidity
Don’t respin serum separator tubes. Serum in contact with RBCs under gel can be expressed and increase which analyte?
K
electrolytes inc. in hemolysis
K, LD
Calibration of centri is done _________.
monthly
Disinfection of centri is done __________.
weekly
Apparatus used for centri calibration
Tachometer
Strobe light
Timer
pipette used to hold a particular volume but not dispense the exact volume
TC
pipette used to dispense exact amount
TD
Automatic pipette that relies on piston for suction
Air disp.
automatic pipette that uses disposable tips and is not suitable for viscous fluids
air disp.
automatic pipette that operates by moving the piston in the pipette tip or barrel, much like hypodermic syringe
Positive disp.
Positive displacement characteristics:
- lack air cushion
- suitable for viscous fluids
- constant aspiration force
Inc or Dec during activity/exercise:
Protein
Inc.
Inc or Dec during activity/exercise:
LD
Inc.
Inc or Dec during activity/exercise:
Lactic acid
Inc.
Inc or Dec during activity/exercise:
Glucose
Dec.