CC Flashcards
the only hypoglycemic hormone
insulin
hyperglycemic hormones
GAGCHETS
Glucagon
ACTH
GH
Cortisol
Human Placental Lactogen
Epinephrine/Norepinephrine
Thyroxine
Somatostatin
glucose to FA
Lipgenesis
glucose to glycogen from storage
glycogenesis
breakdown of glycogen to G6PO4
Glycogenolysis
Hypoglycemia of infancy and childhood
galactosemia
GSD
Reye’s syndrome
Glycogen stores are used up in about ______ hrs
24-48
Decomposition of fat
Lipolysis
Hyperglycemia (level)
> /= 126 mg/dL
brittle diabates
Type 1
Stable diabetes
Type 2
DM assoc. with B cell destruction
Type 1
DM assoc. with insulin resistance
Type 2
DM
C-peptide undetectable
Type 1
DM
C-peptide detectable
Type 2
Normal ratio of C-peptide:insulin
5:1-15:1
BMI:
Obese
> /= 30 kg/m^2
BMI
Overweight
25-29 kg/m^2
BMI
Underweight
<18.5 kg/m^2
the brain can use ketones as a source of energy through what cycle?
TCA
Tricarboxylic acid
3 types of ketone bodies
B hydroxybutyrate
Acetoacetic acid
Acetone
parent ketone
Acetoacetic acid
Major ketone
B hydroxybutyrate
% of parent ketone
20%
% of major ketone
78%
% of least ketont
2%
Ratio of B-hydroxybutyrate to acetoacetate in severe D:M
6:1
Methods to detect ketones
Gerhardt’s ferric chloride test
Nitroprusside test
Acetest
test for ketone bodies which react only with acetoacetate
Gerhardt’s Ferric chloride test
DM associated to HLA DR3 and DR4
Type 1
Interferences in ketone body tests that can cause false (+)
ACE inhibitor
Other sulfhydryl medications
Vitamin C interference in ketone body tests
False (-)
DM electrolyte imbalance
Na, K
Low Na
Increased K
Diabetic nephropathy is also termed as
Kimmel Stiel Wilson
Sodium fluoride preserves glucose up to how many days
3
glucose oxidase method measures the
B-D Glucose
most specific method for glucose measurement
hexokinase
measures the rate of O2 consumption which is equal to glucose conc.
Polarographic glucose oxidase
Enzymes involved in glucose oxidase method
glucose oxidase
peroxidase
enzymes involved in hexokinase method
hexokinase
G6PD
2 type of chemical methods for glucose measurement
alkaline copper reduction
condensation
reagent in Folin Wu method
phosphomolybdate
rgt in Nelson Somogyi
arsenomolybdate
reduction of cupric with glucose (2,9-dimethyl-1-10 phenanthroline hydrochloride)
Neocuproine method
Dubowski method rgt (ortho-Toluidine)
glacial acetic acid
Modification of Folin Wu
Benedict’s
Stabilzing agents for condensation method for glucose measurement
citrate
tartrate
HbA1c reference range
4-6%
HbA1c effective treatment range
<7%
Hemolytic disease can cause falsely ________ HbA1c
low
glycated albumin is also known as
fructosamine
Fructosamine is used to measure glucose control for how long?
3-6 weeks
Liver disease can cause falsely _____ Albumin
low
lab test for glucose homeostasis
FBS
Normal FBS
<100 mg/dL
Impaired plasma glucose
100-125 mg/dL
Diabetes - FBS
> /= 126 mg/dL
Diagnosis criteria for DM
RBS and 2 hr post glucose load = >/= 200 mg/dL
FBS >/= 126 mg/dL
If a mother is not found to have GDM during initial screening. she must be retested at ______ weeks of gestation
24-28
Screening test for GDM
1 hr GCT
Confirmatory test for GDM
OGTT
100g or 75 g (WHO) glucose load
glucose load in 1 hr GCT
50 g
Positive screening test for GDM
> /= 140 mg/dL
GDM is diagnosed if ____ plasma values are above normal level
2
2 methods of GTT
Intravenous
Oral
Methods of OGTT
Janney-Isaac
Exton Rose
OGTT - single dose
Janney-Isaac
OGTT - divided dose
Exton Rose
CSF glucose is collected ____ hr after blood glucose
1
CSF glucose is approx. ____% of plasma concentration
60
most important carb to be tested in newborn
Galactose
Diagnostic test for Galactose-1-phosphate UT
Erythrocyte galactose-1-PO4 uridyl transferase activity
fructokinase catalyzed the conversion of fructose to fructose 1 phosphate
Essential fructosuria
2 methods to test essential fructosuria
Seliwanoff
Bial’s
Fructose test utilizing resorcinol in diluted HCl
Seliwanoff
color rxn of Seliwanoff test
cherry red/red-orange
color rxn of Bial’s test
red
diagnostic test for essential fructosuria:
presence of fructose in urine
GSD muscle forms
2,5,7
GSD hepatic forms
1,3,4,6,11