Isolation & Isomerization Of Lycopene From Tomato Paste Flashcards
What is the [3 part] purpose of this lab?
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Why is it easier to extract lycopene from commercial tomato paste (as compared to a ripe tomato)?
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Why is it important to avoid unnecessary delays & exposure of the pigment to heat or bright light?
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How can we remove the acids that are naturally found in tomatoes?
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Why should you try to record your lycopene solution’s UV-VIS spectrum on the same day of isolating the lycopene solution?
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What solvent mixture will you be using to extract the pigments from the canned Tomato Paste?
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Why are Acetone & Petroleum Ether great solvents to choose for extracting the carotanoid pigments from the Tomato Paste?
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What should you do with the [conc] pigment solution? Which absorbent (stationary phase) are we using in our glass column?
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What is the result of a poorly packed column?
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What should you do after the [conc] pigment solution is at the top?
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What is the speed of each pigment? Why do they have these speeds?
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Trans-lycopene vs. cis-lycopene % UV Spec [just look at the visual]
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How can you estimate the % of all-trans lycopene in your sample?
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What if your % of all-trans lycopene is significantly lower than 90%?
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Why should you compare. Your results with those of the other members of your team?
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