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Organic Chemistry > Column Chromatography (Liquid-Solid) > Flashcards

Column Chromatography (Liquid-Solid) Flashcards

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1
Q

What is the usual stationary phase (in general)?

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2
Q

What is the ‘sample’ and what do we do with it (in general)?

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3
Q

What is the Mobile Phase? What is it’s other name? What does it do (in general)?

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4
Q

Why does separation of the components occur?

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5
Q

What is the difference in meanings between “Adsorbed” and Dissolved”?

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6
Q

What are the 4 separation factors?

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7
Q

Which 2 Adsorbents are the most popular? What is 1 special thing about the first one?

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8
Q

What is the ‘Activity’ of an adsorbent? Why are some more active than others? What is the result (in regards to the bands) of a more active one than a less active one?

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9
Q

Why is it important to keep containers tightly closed and to minimize their exposure to atmospheric moisture?

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10
Q

Which 2 kinds of adsorbents are good for most purposes?

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11
Q

How much adsorbent should be used? What does it depend on? What is the correlation between Polarity Difference and Column Length?

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12
Q

What happens if Particles are too small? If Particles are too LARGE?

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13
Q

How is Particle Size of an adsorbent indicated? What is a Mesh #? What is the correlation between Mesh # vs. Particle Size? What is a typical mesh # for Silica Gel vs. Alumina?

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14
Q

What is the Role of the Mobile Phase?

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15
Q

Why is it best to start with a low polarity Mobile Phase?

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16
Q

Why are several solvents used in sequence, starting with the weaker eluants?

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17
Q

How exactly should you change (throughout the experiment) your solvents used? What is the Rule of Thumb in regards to when to change your solvent?

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18
Q

What does the set-up look like?

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19
Q

How slow should the flow rate be? What exact #s can be used for most purposes? Do difficult separation require slower or faster flow rates?

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20
Q

What are the 3 Keys to packing a column properly?

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21
Q

Why must the adsorbent be kept covered with the solvent at all times?

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Organic Chemistry (4 decks)

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