Isolation and Cultivation Flashcards
Indicative of purity
No. of colonies (>1 = contaminated)
What comprise the dry weight of a cell?
Carbon, nitrogen, phosphorous, vitamins, trace elements
Imprecise nutrition composition
Complex Culture Media
Processes in nitrogen sources
Protein synthesis & amino acid production
Has additional nutrients (eg. blood, serum)
Enriched Medium
CO2 is reduced to glucose
Autotrophic
What is calcium for
Cell wall stability, heat stabilization for endospores
Common elements
P, S, K, Mg
EMB inhibits growth of
Gram +
Contains compounds that inhibit the growth of one bacterial group while supporting the growth of another
Selective Medium
Cofactors in enzymatic reactions
Trace & Vitamins
Transparent outside
Capability to form biofilm
Color of EMB
Green or brown
Use fir liquid media
Preservation
Color of E. Coli
Metallic green
Characteristics that can help in determining unknown organism
Form, margin, elevation, texture, size, color
Whole RBC
Blood Plate Agar
Chocolate agar is used for isolating
Neisseria and H. influenzae
RBC that have broken up
Chocolate Agar
Necessary for synthesis of new cell materials
Carbon sources
Detect biochemical reactions that may occur (by dyes changing color)
Differential Culture Medium
Agar is composed of how much liquid vol.
1.5-3%
Increased enzyme synthesis (uptake of limited nutrients, replacement of limited in place of available)
Adaptation
Prefers glucose, lactose
Heterotrophic
Also detects lactose fermentation
EMB
Blood plate agar is used to isolate
S. haemolyticus (which is infectious and has different variants)
Most preferred by certain bacteria
Glucose
MSA is composed of (blank) and detects (blank)
Phenol red, mannitol fermentation
Use for solid media
Isolation & purification
MacConkey (MAC) agar is composed of (blank) and inhibits (blank)
Bile salts, Gram +
Precise amounts of highly purified organic/inorganic components
Defined Culture Media
Complex culture media is usually in the color
Yellow
Ccomposition of MAC and what does it detect
Neutral red, lactose fermentation
Decrease in metabolic activity
Adaptation
13% of the cell
Nitrogen sources: proteins, nucleic acid, etc.
Composed of 7.5% NaCl and inhibits Gram - & salt sensitive Gram +
Mannitol Salt Agar (MSA)
Where to get nitrogen
Tryptone, peptone
True or false. Sodium content depends in the nutrient requirement of the bacteria.
True.
