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MICROBIO LEC > Isolation and Cultivation > Flashcards

Isolation and Cultivation Flashcards

1
Q

Indicative of purity

A

No. of colonies (>1 = contaminated)

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2
Q

What comprise the dry weight of a cell?

A

Carbon, nitrogen, phosphorous, vitamins, trace elements

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3
Q

Imprecise nutrition composition

A

Complex Culture Media

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4
Q

Processes in nitrogen sources

A

Protein synthesis & amino acid production

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5
Q

Has additional nutrients (eg. blood, serum)

A

Enriched Medium

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6
Q

CO2 is reduced to glucose

A

Autotrophic

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7
Q

What is calcium for

A

Cell wall stability, heat stabilization for endospores

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8
Q

Common elements

A

P, S, K, Mg

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9
Q

EMB inhibits growth of

A

Gram +

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10
Q

Contains compounds that inhibit the growth of one bacterial group while supporting the growth of another

A

Selective Medium

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11
Q

Cofactors in enzymatic reactions

A

Trace & Vitamins

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12
Q

Transparent outside

A

Capability to form biofilm

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13
Q

Color of EMB

A

Green or brown

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14
Q

Use fir liquid media

A

Preservation

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15
Q

Color of E. Coli

A

Metallic green

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16
Q

Characteristics that can help in determining unknown organism

A

Form, margin, elevation, texture, size, color

17
Q

Whole RBC

A

Blood Plate Agar

18
Q

Chocolate agar is used for isolating

A

Neisseria and H. influenzae

19
Q

RBC that have broken up

A

Chocolate Agar

20
Q

Necessary for synthesis of new cell materials

A

Carbon sources

21
Q

Detect biochemical reactions that may occur (by dyes changing color)

A

Differential Culture Medium

22
Q

Agar is composed of how much liquid vol.

A

1.5-3%

23
Q

Increased enzyme synthesis (uptake of limited nutrients, replacement of limited in place of available)

A

Adaptation

24
Q

Prefers glucose, lactose

A

Heterotrophic

25
Q

Also detects lactose fermentation

A

EMB

26
Q

Blood plate agar is used to isolate

A

S. haemolyticus (which is infectious and has different variants)

27
Q

Most preferred by certain bacteria

A

Glucose

28
Q

MSA is composed of (blank) and detects (blank)

A

Phenol red, mannitol fermentation

29
Q

Use for solid media

A

Isolation & purification

30
Q

MacConkey (MAC) agar is composed of (blank) and inhibits (blank)

A

Bile salts, Gram +

31
Q

Precise amounts of highly purified organic/inorganic components

A

Defined Culture Media

32
Q

Complex culture media is usually in the color

A

Yellow

33
Q

Ccomposition of MAC and what does it detect

A

Neutral red, lactose fermentation

34
Q

Decrease in metabolic activity

A

Adaptation

35
Q

13% of the cell

A

Nitrogen sources: proteins, nucleic acid, etc.

36
Q

Composed of 7.5% NaCl and inhibits Gram - & salt sensitive Gram +

A

Mannitol Salt Agar (MSA)

37
Q

Where to get nitrogen

A

Tryptone, peptone

38
Q

True or false. Sodium content depends in the nutrient requirement of the bacteria.

A

True.

MICROBIO LEC (6 decks)

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