ISBB Flashcards
To reinactivate the complement system,
serum is heated at what temperature and
interval if a modified VDRL testing involving
macroscopic agglutination is used?
a. 56 C for 30 minutes
b. 56 C for 10 minutes
c. 65 C for 30 minutes
d. None of the above
None of the above
A lack of C1-INH might result in which of the
following conditions?
a. PNH
b. Atherosclerosis
c. HANE
d. Increased bacterial infection
HANE
A type of immunization where a
microorganism loses its pathogenicity but
retains its capacity for transient growth?
a. Live attenuated vaccine
b. Toxoid vaccine
c. Inactivated vaccine
d. Polysaccharides
Live attenuated vaccine
Which of the following is associated with
defective killing by phagocytes?
a. CGD
b. SCID
c. Di George Anomaly
d. WAS
CGD
These antibodies are the marker for SLE and
the antibodies are associated with
active/severe disease and has been found
almost exclusively in patients with SLE.
Although they are found in only 25-30% of
patients, the presence of these antibodies is
considered diagnostic for SLE; the antibodies typically produce a speckled
staining pattern in FANA/IIF.
a. Anti-dsDNA
b. Anti-sm
c. Anti-RNP
d. Anti-DNP
e. Anti-nucleolar
Anti-sm
Which of the following markers is used for
the identification of NK cells and mediates
phagocytosis and ADCC?
a. CD4
b. CD8
c. CD16
d. CD56
CD16
How much diluent needs to be added to
0.25 ml of serum to make a 1:20 dilution?
a. 0.5 mL
b. 4.75 mL
c. 3.8 mL
d. 5 mL
4.75 mL
In an antibody titration, a 0.2mL aliquot of a
patient’s serum sample was added to 0.8mL
of saline, and this mixture was placed into
tube #1. A 0.5mL sample was removed from
tube 1 and placed into tube 2, containing
0.5mL of saline. This procedure was
repeated through tube #10. How should the
antibody titer be reported if the last positive
reaction was observed in tube #10?
a. 640
b. 2560
c. 5120
d. 10240
5120
The serum of an individual who received all
doses of the hepatitis B vaccine should
contain:
a. Anti-Hbs
b. Anti-Hbe
c. Anti-HBc
d. HBsAg
Anti-Hbs
Which of the following is indicative of a
recent infection with T. gondii?
a. Anti-Toxoplasma IgM
b. Anti-Toxoplasma IgE
c. High avidity anti-Toxoplasma IgG
d. Low avidity anti-Toxoplasma IgG
d. Low avidity anti-Toxoplasma IgG
Which of the following is indicative of a
parasitic infection?
a. Increased IgA levels
b. Increased IgE levels
c. Increased IgG levels
d. Increased IgM levels
Increased IgE levels
A person has an infected bug bite with pain,
swelling, and redness. What is the cause of
these physical symptoms of inflammation?
a. Production of antibody
b. Secondary immune response
c. Increased blood flow and neutrophils
to site
d. Activation of NK cells
Increased blood flow and neutrophils
to site
What are the associated autoantibodies in
autoimmune hepatitis?
a. Anti-smooth muscle antibody
b. Anti-liver kidney microsomal antibody
c. Anti-liver cytosol type 1 antibody
d. All of the above
All of the above
Destruction of the myelin sheath of axons
caused by the presence of antibody is
characteristic of which disease?
a. MS
b. CGD
c. MG
d. GPS
MS
What is the basic difference between the
RPR and VDRL tests?
a. The RPR detects antigen, whereas the
VDRL detects antibody.
b. The RPR test is read macroscopically,
whereas the VDRL is read
microscopically.
c. The RPR test is a treponemal test,
where the VDRL is nontreponemal.
d. There is no difference because they are
both non-specific test for syphilis
The RPR test is read macroscopically,
whereas the VDRL is read
microscopically.
What is the difference between
nephelometry and turbidimetry?
a. There is no difference between two
assays.
b. Nephelometry is newer example of
turbidimetry.
c. Nephelometry measures light
transmitted through a solution, and
turbidimetry measures light scattered in
a solution.
d. Nephelometry measures light scattered
in a solution, and turbidimetry
measures light transmitted through a
solution.
Nephelometry measures light scattered
in a solution, and turbidimetry
measures light transmitted through a
solution.
Postzone causes false-negative reactions in
antibody titers. What is the remedy to
resolve this problem?
a. Excess antigen in test
b. Excess antibody in test
c. Repeat the test after a week
d. Both A and B
e. Both A and C
Repeat the test after a week
Which Immunoglobulin best initiate the
classic complement pathway?
a. IgA
b. IgG
c. IgM
d. Both A and C
e. Both B and C
IgM
Which agglutination test in detecting
syphilis uses glutaraldehyde-stabilized
turkey RBCs?
a. HATTS
b. TPHA
c. MHA-TP
d. TPPA
HATTS
Which of the following would represent a
double negative thymocyte?
a. CD2- CD3+ CD4- CD8+
b. CD2- CD3- CD4+ CD8-
c. CD2+ CD3+ CD4- CD8-
d. CD2- CD3- CD4+ CD8+
CD2+ CD3+ CD4- CD8-
What B cell maturation stage is
characterized by the presence of IgM and
IgD on the cell’s cytoplasm?
a. Plasma cells
b. Mature B cells
c. Immature B cells
d. Pre-B cell
Plasma cells
Type IV hypersensitivity reaction:
I. Contact dermatitis
II. Jewelry and latex
III. Poison Ivy
IV. SLE
V. Peanut allergies
a. I, II, III
b. I, II, III, IV
c. I, II, III, IV, V
d. I, II, III, V
e. I and III
I, II, III
A positive result in Agglutination inhibition
test is indicated by:
a. Presence of agglutination
b. Absence of agglutination
c. Presence of hemolysis
d. Absence of hemolysis
Absence of agglutination
A positive result in Complement Fixation
test is indicated by:
a. Presence of agglutination
b. Absence of agglutination
c. Presence of hemolysis
d. Absence of hemolysis
Absence of hemolysis
MHC class I-deficient diseased cells trigger
and activate:
a. B cells
b. T cells
c. NK cells
d. Interferon
NK cells
Hybridoma is produced from the fusion of:
a. T cells and plasma
b. B cells and plasma
c. Myeloma and plasma cells
d. Myeloma and T cells
Myeloma and plasma cells
Reverse passive agglutination:
I. Antigen is attached to a carrier
particle.
II. Antibody is attached to a carrier
particle. III. Agglutination occurs if the patient
antibody is present.
IV. Agglutination occurs if the patient
antigen is present.
a. I, III
b. II, IV
c. I, IV
d. II, III
II, IV
. RF is associated with which immunoglobulin
classes?
a. IgM
b. IgG
c. IgA
d. All of the above
All of the above
Which of the following does not
characterize T cell function?
a. Produce and secrete immunoglobulins
b. Develop killer cells that produce
cytokines
c. Suppress the immune response
d. Develop helper cells
Produce and secrete immunoglobulins
During the “window phase” of HBV
infection, which of the following may be the
only detectable marker?
a. Anti-HBc
b. Anti-HBe
c. Anti-HBs
d. HBsAg
Anti-HBc
Which of the following statements
regarding immunogenicity is true:
a. The immunogen must be recognized by
the body as nonself.
b. The immunogen must be greater than
10 Da.
c. Proteins and carbohydrates are the
most immunogenic, whereas lipids and
nucleic acids are weakly immunogenic.
d. The more complex a molecule, the
more immunogenic it becomes
The immunogen must be greater than
10 Da.
The interaction between individual antigen
and antibody molecules depends on several
types of bonds, such as ionic bonds,
hydrogen bonds, hydrophobic binds, and
van der Waals forces. How is the strength of
this attraction characterized?
a. Avidity
b. Affinity
c. Reactivity
d. Valency
Affinity
What disease is indicated by a high titer of
anti-Sm antibody?
a. MTCD
b. RA
c. SLE
d. Scleroderma
SLE
Which immunoglobulin can cross the
placenta? *
a. IgG
b. IgM
c. IgA
d. IgE
IgG
Which of the following is not a type 1
hypersensitivity reaction? *
a. Asthma
b. Hay Fever
c. Serum Sickness
d. Urticaria
Serum Sickness
Which of the following is associated with
MHC class I? *
a. HLA-A, B, C
b. Exogenous
c. Endogenous
d. A and B
e. A and C
Which CD4:CD8 ratio is most likely in a
patient with AIDS? *
a. 1:2
b. 2:1
c. 1.5:1
d. 1:1.5
1:2
Antigenic groups identified by different sets
of antibodies reacting in a similar manner to
certain standard cell lines best describes:
a. Cytokines
b. CD
c. Neutrophil granules
d. Opsonin
CD
The action of CRP can be distinguished from
that of an antibody because:
a. CRP acts after the antibody appears
b. Only the antibody triggers the
complement cascades
c. Binding the antibody is calcium
dependent
d. CRP acts before the antibody appears
CRP acts before the antibody appears
When combining acid and water, *
a. Acid is added to water
b. Water is added to acid
c. Water is slowly added to acid
d. Both solutions are combined
simultaneously
Acid is added to water
If a serological test is positive for an
individual who does not have a particular
disease, the result was caused by a problem
with:
a. Sensitivity
b. Specificity
c. Accuracy
d. Poor pipetting
Specificity
Which technique represents a singlediffusion reaction?
a. RID
b. Ouchterlony diffusion
c. Immunoelectrophoresis
d. Immunofixation electrophoresis
RID
Which of the following correctly describes
reverse passive agglutination?
a. It is a negative test.
b. It can be used to detect autoantibodies.
c. It is used for identification of antigens.
d. It is used to detect sensitization of RBC.
It is used for identification of antigens.
Which of the following statements
accurately describes competitive binding
assays?
a. Excess binding sites for the analyte are
provided.
b. Labeled and unlabeled analyte are
present in equal amounts.
c. The concentration of patient analyte is
inversely proportional to bound label.
d. All the patient analyte is bound in the
reaction
The concentration of patient analyte is
In a noncompetitive enzyme immunoassay,
if a negative control shows the presence of
color, which of the following might be a
possible explanation?
a. No reagent was added.
b. Washing step were incomplete.
c. The enzyme was inactivated.
d. No substrate was present.
Washing step were incomplete.
. Which of the following would support the
diagnosis of drug-induced lupus?
a. Antihistone antibodies
b. Antibodies to smith antigen
c. Presence of RF
d. Antibodies to SS-A and SS-B antigens
Antihistone antibodies
The process whereby the leukocyte
squeezes in ameboid fashion across the
endothelial cells. This almost always
happens at endothelial cell borders.
a. Chemotaxis
b. Vasodilation
c. Transmigration
d. None of the above
Transmigration
. Colostrum:
a. Active natural immunity
b. Active artificial immunity
c. Passive natural immunity
d. Passive artificial immunity
Passive natural immunity
In primary biliary cirrhosis, which of the
following antibodies is seen in high titers?
a. Antimitochondrial
b. Anti-smooth muscle
c. Anti-DNA
d. Anti-parietal cell
Antimitochondrial
Live attenuated vaccine:
a. Rabies
b. Tetanus
c. Hepatitis B
d. Measles
Measles
What kind of antigen-antibody reaction
would be expected if soluble antigen is
added to homologous antibody?
a. Precipitation
b. Agglutination
c. Complement fixation
d. Hemagglutination
Precipitation
A specific component of the adaptive
immune system that is formed in response
to antigenic stimulation: *
a. Lysozyme
b. Complement
c. Commensal organisms
d. Immunoglobulin
Immunoglobulin
Anti-glomerular basement membrane
antibody is most often associated with this
condition: *
a. Goodpasture disease
b. SLE
c. Celiac disease
d. Chronic active hepatitis
Goodpasture disease
This is a surrogate testing for HBV that is no
longer required. *
a. ALT
b. HBsAg
c. Anti-HBc
d. Anti-HBsAg
ALT
Which WBC is not an end cell? *
a. B cell
b. Neutrophil
c. Eosinophil
d. Basophil
e. Monocyte
Monocyte
T cells are incapable of functioning as: *
a. Cytotoxic cells
b. Helper cells
c. Phagocytic cell
d. Regulatory cell
Phagocytic cell
Antigen receptor on T cells bind HLA class II
molecules with the help of which accessory
molecule? *
a. CD2
b. CD3
c. CD4
d. CD8
CD4
Flocculation test for syphilis detects the
presence of: *
a. Reagin
b. anticardiolipin
c. Cardiolipin
d. Both A and B
e. Both A and C
Both A and B
What is the purpose of the hemolytic
system in a complement fixation test?
a. To test the patient’s serum for the
presence of antibodies
b. To act as an indicator and provide a
visible reaction
c. To test the red blood cells
d. To make certain that the serum is not
anticomplementary
To act as an indicator and provide a
visible reaction
What is the diameter of the ceramic ring in
mm used for quantitative VDRL?
a. 14
b. 15
c. 16
d. 18
14
The quantitative VDRL test is performed on
all sera in which qualitative VDRL test?
a. Nonreactive
b. Reactive
c. Weakly reactive
d. Either reactive or weakly reactive
either reactive or weakly reactive
The reactions on DNA-methyl green
substrates when testing for the presence of
anti-DNase are: POSITIVE- NEGATIVE
A. Blue Yellow
B. Red Orange
C.Green Colorless
D.Purple Yellow
Green Colorless
Forssman antibodies are absorbed by:
a. Boiled beef cells
b. Guinea pig kidney antigen
c. Neither beef cells nor guinea pig kidney
antigen
d. Both beef cells and guinea pig kidney
antigen
Guinea pig kidney antigen
The Weil-Felix test is used for the detection
of which type of antibodies?
a. Salmonella
b. Mycoplasma
c. Rickettsia
d. Viral
Rickettsia
What is the gold standard test for the
detection of VZV antibody?
a. FAMA
b. IFA
c. Sabin-Feldman Dye Test
d. Culture
FAMA
How many molecules of IgM are needed to
fix complement?
a. 1
b. 2
c. 3
d. 4
In Direct Immunofluorescent assay, ___ that
is conjugated with a fluorescent tag is added
directly to unknown ____ that is fixed to a
microscope slide.
a. Antigen, antibody
b. Antibody, antigen
c. Enzyme, antigen
d. Enzyme, antibody
Antibody, antigen
Which of the tests is/are correctly matched?
*
a. Southern: DNA
b. Southern: RNA
c. Northern: DNA
d. Northern: RNA
Southern: DNA
Which serum antibody response usually
characterizes the primary stage of syphilis?
*
a. Antibodies against syphilis are
undetectable
b. Detected 1-3 weeks after appearance
of primary chancre
c. Detected in 50% of cases before the
primary chancre disappears
d. Detected within 2 weeks after infection
Detected 1-3 weeks after appearance
of primary chancre
Which tests are considered screening test
for HIV? *
a. ELISA, 4th generation, and rapid
antibody tests
b. Immunofluorescence, western blot,
radioimmuno-precipitation assay
c. Culture, antigen capture assay, DNA
amplification
d. Reverse transcriptase and messenger
RNA assay
ELISA, 4th generation, and rapid
antibody tests
0.2 mL of serum is transferred to tube # 1
and 3.8 mL of saline is added to dilute the sample. Determine the Dilution Factor for
tube number # 1. *
a. 3.6 mL
b. 3.8 mL
c. 1:20
d. 1:10
e. 1:4
1:20
Cell-mediated adaptive immunity: *
a. T cell
b. B cell
c. Dendritic cell
d. NK cell
T cell
Immune complex: *
a. Type I
b. Type II
c. Type III
d. Type IV
Type III
What region differentiates various classes of
immunoglobulin? *
a. Constant region of the heavy chain
b. Variable region of the heavy chain
c. Constant region of the light chain
d. Variable region of the light chain
Constant region of the heavy chain
True about Sabin-Feldman Dye Test, except:
*
a. Measures IgG antibodies
b. Its principle involves neutralization
c. Makes use of living organism
d. Positive result: stained deeply blue
with methylene blue
Positive result: stained deeply blue
with methylene blue
What is the most common complement
deficiency?
a. C1
b. C2
c. C3
d. C5
C2