IS Lab Finals - Radioimmunoassay (RIA)

Question 1

An older and less frequently used laboratory technique using radioactive substances to evaluate immunoglobulins.

Answer 1

Radioimmunoassay

Question 2

done with specific antibodies in liquid solution

Answer 2

Traditional RIA

Question 3

uses antibody bound to a solid support (e.g. tube, glass beads)

Answer 3

Solid-phase RIA

Question 4

Who developed Radioimmunoassay? in what year?

Answer 4

Yalow and Berson (1959)

Question 5

Most commonly performed nuclear medicine technique

Answer 5

Radioimmunoassay

Question 6

Uses a radioactive label that could identify an immunocompetent at very low concentrations

Answer 6

Radioimmunoassay

Question 7

what is the principle of RIA?

Answer 7

Competitive immunoassay

Question 8

what is the label used in RIA?

Answer 8

radioactive substance

Question 9

have nuclei that decay spontaneously, emitting matter and energy

Answer 9

Radioactive elements

Question 10

Most commonly used radioisotope

Answer 10

Iodine-125

Question 11

Easily incorporated into protein molecules and emits gamma radiation

Answer 11

Iodine-125

Question 12

what is used to measure I-125?

Answer 12

gamma counter

Question 13

2 advantages of RIA

Answer 13

• Extremely sensitive
• Precise technique for determining trace amounts of analytes that are small in size

Question 14

4 disadvantages of RIA

Answer 14

• Health hazard
• Disposal problems
• Short shelf life
• Requires expensive equipment

Question 15

3 applications of RIA

Answer 15

• Detecting tumor markers
• Measures insulin and glucose estimation
• Detection and measurement of different hormones

Question 16

7 hormones measured by RIA

Answer 16

• Growth hormone
• Cortisol
• Aldosterone
• Sex hormones
• Prolactin
• Gonadotropin
• Parathyroid hormone