Investigation Into Photosythesis Flashcards
1
Q
How do u extract photosynthetic pigments
A
Use a pestle and mortar to grind leaf with a extraction solvent
2
Q
Outline how paper chromatography can be used to separate photosynthetic pigments
A
- use capillary tube to spot pigment extract onto pencil start line. Cm able bottom of paper
- place chromatography pepper in solvent
- allow spent to run until it almost touches the other end of the per. Pigments moe different distances
3
Q
What is RF values
A
Ratios that allow compares of how far molecules have moved in chromatograms
4
Q
How to calculate RF value
A
Instance between origin and centre f pigment spot/ distance between origin and Solvent front
5
Q
Purpose and principle of paper chromatography
A
Molecules in a mixture are separated based on their relative attraction to the mobile phase vs the stationary phase