investigating enzymatic reactions

Question 1

amylase catalyses …..

Answer 1

the breakdown of starch to sugar

Question 2

how can you detect starch

Answer 2

using iodine solution

Question 3

if starch is present the solution will change from

Answer 3

browny orange to blue black

iodine

Question 4

what are the steps of testing for starch

Answer 4

1) put drop of iodine into spotting tile
2) set up a bunsen burner with a water beaker
3) add amylase solution and buffer solution with ph 5 into boiling tube
4) put tube into beaker and wait for 5 mins
5) add starch solution to boiling tube
6) every 30 secs take a sample and drop into a well in spotting tile
7) when iodine solution stays brown all starch in sample has been broken down

Question 5

what should you do once you’ve completed the first experiment

Answer 5

repeat at different ph

Question 6

how do you measure the rate of reaction

Answer 6

rate = 1000/time