Investigating Antibiotic Resistance

Question 1

What are the variables to consider?

Answer 1

Discs must be same size
Same volume and concentration of chemical
Discs must be placed evenly spaded and not too close to the edge
Control disc should be used one that has been soaked in just water to demonstrate it is the antibiotic in the the discs having an affect

Question 2

What are the general aseptic techniques?

Answer 2

Wash hands
Disinfect workplace
Light Bunsen burner on workplace

Question 3

What is the first step?

Answer 3

loosen lid of the bottle contain bacterial culture and flame the neck of the bottle

Question 4

What is the second step?

Answer 4

Use a sterile pipette to draw a small amount, flame the neck of bottle and close it

Question 5

What is the third step?

Answer 5

partially lift lid of petri dish, place a few drops, close lid and place pipette in a discard jar of disinfectant

Question 6

What is the fourth step?

Answer 6

Dip a glass spreader into 70% alcohol, pass quickly through the Bunsen burner flame to ignite the alcohol

Question 7

What is the fifth step?

Answer 7

Lift lid of petri dish slightly and in zig zag motion spread the alcohol

Question 8

What is the sixth step?

Answer 8

place lid on petri dish and flame the spread using alcohol

Question 9

What is the seventh step?

Answer 9

place discs onto the agar using forceps

Question 10

What is the eighth step?

Answer 10

Tape petri dish close and store upside down, 25C