Introduction To The Use Of Microscopes

Question 1

Clinical relevance

Answer 1

To distinguish normal tissue from abnormal tissue.

Identify source of abnormal tissue; best possible treatment

Question 2

Steps

Answer 2

Fixation
Sectioning
Shape
Size
Stain

Question 3

H and E stain

Answer 3

Hematoxylin and eosin

Haematoxylin:
Basophilic; basic dye;stains acid components (nuclei) purple-blue

Eosin:
Acidophilic; acidic dye; stains basic components pink (cytoplasm)

Question 4

PAS stain

Answer 4

Periodic Acid Schiff

Oxidase should sugars and stains resulting aldehydes bright pink-purple

Question 5

Trichrome stain

Answer 5

Used to show up different components

Question 6

Limit of resolution

Answer 6

Light microscope - 0.2 micrometers (highest useful magnification is 1500
Electron microscope - 0.2 nm

Question 7

Size of:
Ovum
Most cells, RBCs
Nuclei
Mitochondria
Ribosomes

Answer 7

80-140 micrometers (naked eye)
7-20 micrometers (LM) ; 7.2 micrometers
7-12 micrometers (LM)
2 micrometers * 0.2 micrometers (LM)
15 nm (EM)

Question 8

Methods to study cell structure and function:
Light microscopy:
Electron microscopy:

Answer 8

-Phase contrast
Fluorescence
Confocal

• Transmission
  Scanning

Question 9

What structures would tell whether it is a secretory tissue?

Answer 9

Zymogen granules
Rough endoplasmic reticulum
Nucleus with prominent nucleolus