Introduction to Micro anatomy

Question 1

Tissue Preparation: Fixation

Answer 1

Purpose: to preserve tissues and prevent tissue degredtion

Small pieces placed into chemial solutions

Large organs or whole bodies have blood vessels infused with chemicals

Question 2

Fixative for light microscopy

Answer 2

Formalin

Question 3

Fixative for electron microscopy

Answer 3

glutaraldehyde

Question 4

Tissue Preparation: Embedding and Sectioning

Answer 4

Purpose: to allow tissue to be sliced thinly enough to be examined by microscopy

Question 5

Embedding for light microscopy

Answer 5

Paraffin

Question 6

Embedding for electron microscopy

Answer 6

plastic resins

Question 7

Tissue preparation: Staining

Answer 7

Purpose: allows cells and ECM to be seen

Question 8

H&E stain

Answer 8

Routine Exam

Hematoxylin is a dark blue voilet stain. Is basophilic/ positive. Binds to acidic/ - such as DNA/RNA

Eosin is red/pink counterstain. Is acidic/ -. Binds to acidophiolic + substances such as cytoplasmic proteins

Question 9

Gram Stain

Answer 9

To determine if/ what kind of bacteria present in a substance.

Tissue stained with crystal viollet dye and Grams iodine solution, Then decolorized and counterstained.

Gram + violet bc thick peptidoglycan wall

Gram - take up pink counteratain

Question 10

Wright Geimsa Stain

Answer 10

To study blood cells.

Combo of stains including methylene blue, azure B, and eosin

Eosin stains cytoplasm pink

Methylene blue and Azure B stain nucleus and components varying shades of blue and purple

Question 11

Immunohistochemistry

Answer 11

Uses labeled antibodies.

Most often to investigate infection and neoplasia

Brown stain