Introduction/ Cell Physio

Question 1

• Anemia?
• Hemostasis?
• Thrombosis?
• 5 types of WBC’s?
• Platelets made from? Ratio?
• Leukemia?
• Lymphoma?
• Acute/Chronic leukemia?
• Lymphoid/ myeloid leukemia?

Answer 1

• Decreased red cell mass
• Stop bleeding
• Blod clot in vessel
• Monocytes, lymphocytes, N, B, E
• Megakaryocytes, 1: 5k
• Cancer in blood or bone marrow
• Cancer outside bone/blood
• Immature cells (rapid), more mature cells
• lymphocyte lineage, other marrow cell types

Question 2

• Wright Geisma stain? Stains basic elements what color? Ex? (3) Stains acidic elements? Ex? (3)
• Granulocytes? (3)
• Total % N? Absolute WBC volume?
• N: Abundance? Function? Absolute count?
• E: Function? Hypersensitivity? Absolute count?
• B: Function? Absolute count?
• Mono: Size? Function? Absolute count?
• Lymph: 2 types? Absolute count?
• Which change with age?

Answer 2

• Stain peripheral smear; Eosin, red-orange, Hb, proteins, granules; Methyl blue, blue, DNA, RNA, cytoplasm
• N, E, B
• 50%, 250 uL
• Most; infection; 2k-8k/uL
• Defend helminthic infection; Type 1; 0-400/uL
• Type 1 Hypersensitivity; 0-200/uL
• Biggest WBC, phagocytize; 200-900/ uL
• Small round or large granular; 1k-5k/uL
• N, Mono, Lympho

Question 3

• Reticulocytes: How long in bone marrow? In blood with RNA and Ribosomes?
• RBC: In men? In women? Decreases when? What about platelets with age and sex?
• Plasma? Tubes? with what?
• Serum? Tubes?

Answer 3

• 3-4 days; 1-2 days
• 5X10^12 /L; 4x10^12 / L; First few months; same by age and sex
• Fluid portion of blood with additive to prevent clots; Purple/Blue
• Clear fluid after clots; Red/gold

Question 4

• Leukocytes?
• Mononuclear cells?
• Dendritic?
• Polymorphonuclear (PMN’s)?
• Mast cells? Similar to?
• Central lymphoid organs?
• Peripheral organs? (5) Lymphocytes enter where?
• Antigen recognized by?
• Immunogen?
• Toleragen?

Answer 4

• Nucleated cells of blood
• Mono/lympho
• Connect innate and adaptive
• B,E,N
• Granules of histamine, similar to basophil
• Thymus and Bone Marrow
• Nodes, spleen, Peyers patch, tonsils, adenoids; post cap venules
• Immune system
• Causes immune response
• No immune response

Question 5

• Humeral immunity? Where? B cells are? Transferred in serum?
• Cell mediated immunity? Transferred in serum?
• Automated WBC count not good with?
• Flow cycrometry measures?
• HCT eq?
• How to prevent clotting?
• MCV =?
• MCH =?
• MCHC =?

Answer 5

• Ab response; extracellular where bacteria live; yes
• T lympho; no
• Abnormal cells
• WBC count
• RBC * MCV / 10
• Add sodium citrate
• (HCT/RBC) * 10
• (HgB/RBC) * 10
• (HgB/HCT) * 10