Introduction Flashcards

1
Q

Class Amphibia contains what three clades? Describe each clade.

A

Gymnophiona - Caecilians. Legless, burrowing amphibians found in wet, tropical areas. Resemble earthworms.
Caudata - Salamanders. Includes sirens (eel-like), basal (primitive) salamanders, and derived salamanders (axolotls, newts, and many terrestrial species)
Anura - Frogs.

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2
Q

Describe salamanders and newts.

A

Salamanders have lungs and numerous poison glands in skin.
Newts can have an aquatic larval stage, terrestrial juvenile period (efts), and aquatic adult stage.

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3
Q

The family Pipidae contains what frogs?

A

Xenopus laevis (xenopus genera) and X. tropicalis (Silurana)

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4
Q

What family do toads belong to? What are characteristics of toads?

A

Bufoniade.
Warty, thick skin with a well-developed parotid glands. Males have a Bidder’s organ (ovarian tissue located on cranial pole of testis)

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5
Q

Define aposomatism.

A

Bright color patterns alert would-be predators to the presence of highly toxic alkaloid skin secretions.

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6
Q

How do poison dart frogs produce their poisons?

A

Alkaloid secretions derived from wild diets of arthropods and greatly reduced in captive frogs eating typical fruit fly and springtail diets.

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7
Q

What frogs belong to the family Ranidae?

A

“True frogs.” Rana/Litohbates, including L. castesbeainus (bullfrogs) and L. pipiens (northern leopard frog).

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8
Q

How does the tympanum of Ranidae frogs differ by sex?

A

Larger than the eye in males, similar to eye diameter in females.

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9
Q

How was X. laevis initially used in research?

A

Pregnancy assays - Injection of a pregnant woman’s urine into the dorsal lymph sac of a female frog caused the frog to begin laying eggs.

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10
Q

Why is Xenopus popular among developmental biologists?

A

Ability to reproduce year-round when injected with commercially available hormones.

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11
Q

Currently, what are Xenopus laevis most commonly used for? What does FETAX stand for?

A

Cell and molecular biology research. Frog Embryo Teratogenesis Assay: Xenopus.

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12
Q

What are advantages to the use of X. tropicals in research?

A

Small genome (10 chromosomes) and short generation time.

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13
Q

What is the scientific name of the axolotl? How are axolotls and newts unique?

A

Ambystoma mexicanum. Regenerate limbs, tail, jaws, skin, spinal cord, brain, and heart apex.

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14
Q

What species do Ranidae frogs (bullfrogs and leopard fronts) belong to? What are they used to research? Why?

A

Lithobates. Antiemetic work due to ability to regurgitate easily.

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15
Q

Where should amphibians be sourced from?

A

Captive populations, or wild populations of invasive species.

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