intro to urinalysis Flashcards
Wrote a book on uroscopy (5th Century BC)
Hippocrates
Developed in 1140 CE to describe the significance of 20 different colors of urine
Color charts
Discovered albuminuria by boiling urine (1694)
Frederick Dekkers
Developed methods of quantitating microscopic sediments (1925)
Thomas Addis
Introduced the concept of urinalysis as part of a doctor’s routine patient examination (1827)
Richard Bright
Ultrafiltrate of the plasma
Urine
Organic components of urine
Urea, Creatinine, Uric Acid
Major inorganic component of urine
Chloride
Normal daily urine output
1200-1500mL
Decrease in urine output
Oliguria
Cessation of urine flow
Anuria
Increase in the nocturnal excretion of urine
Nocturia
Increase in the daily urine volume
Polyuria
Increased or Decreased SG: Diabetes mellitus
Increased
Increased or Decreased SG: Diabetes insipidus
Decreased
Affected by the number of molecules in the solution
Specific Gravity
What are the characteristics of an ideal urine specimen container?
Clean, clear, wide mouthed, dry, leak proof, disposable container, 50mL capacity
Specimen volume needed for analysis
12mL
T/F: Specimen should be delivered to the laboratory promptly and tested within 3 hours.
False. Should be within 2 hours.
T/F: Specimen should be refrigerated (2 to 8C) or have appropriate chemical preservative when there is delay in processing.
True
Increased or Decreased Clarity: Bacterial growth
Decreased
Increased or Decreased Odor: Bacterial multiplication or breakdown of urea to ammonia
Increased
Increased or Decreased pH: Breakdown of urea to ammonia by urease-producing bacteria/loss of CO2
Increased
Increased or Decreased Glucose: Glycolysis and bacterial use
Decreased
Increased or Decreased Ketones: Volatilization and bacterial metabolism
Decreased
Increased or Decreased Bilirubin: Exposure to light/photooxidation to biliverdin
Decreased
Increased or Decreased Urobilinogen: Oxidation to urobilin
Decreased
Increased or Decreased Nitrite: Multiplication of nitrate-reducing bacteria
Increased
Increased or Decreased RBC, WBC, & Casts: Disintegration in dilute alkaline urine
Decreased
Increased or Decreased Bacteria: Multiplication
Increased
Most commonly received specimen
Random Specimen
Ideal screening specimen
First Morning Specimen
Used for quantitative testing
Timed Specimen
Specimen for creatinine clearance
24 hour urine
Specimen for amylase testing
2 hour urine (2AM)
Specimen for urobilinogen testing
2-hour urine (2PM)
Specimen for nitrite determination
4-hour urine
Collected under sterile conditions by passing a hollow tube (catheter) through the urethra into the bladder
Catheterized specimen
Safer less traumatic method for obtaining urine; less contaminated by epithelial. ells and bacteria
Midstream clean-catch specimen
External introduction of a needle thorugh the abdomen
Suprapubic Aspiration
Three-glass collection
Prostatitis specimen
3 glasses in prostatitis specimen
1: first urine passed (sterile)
2: Midstream portion
3: Urine with Prostate fluid
Four-glass urine collection technique
Stamey-Mares Test for Prostatitis
T/F: 3rd specimen will have WBC/hpf and bacterisl count 10x that of 1st specimen
True
What is 2nd specimen used for
Control for kidney and bladder infection
Uses soft clear plastic bags with hypoallergenic skin adhesive for collection
Pediatric specimen
Most vulnerable part of a drug-testing program
Urine collection
Within how many minutes from the time of collection must urine temperature be taken
4 mins
Range of urine temperature
32.5-37.7C
Process that involves documentation of proper specimen identification from the time of collection to the receipt of laboratory results
Chain of Custody
Second voided urine after a period of fasting
Fasting specimen
Urine collected 2 hours after eating
2-hr post prandial specimen