Intro to section 3

Question 1

Is staining the preferred way to see living, moving microbes? & Why?

Answer 1

no, the staining procedures or the stains themselves can kill most microbes

Question 2

Dyes that are positively charged are attracted to the ___ components of living cells?

Answer 2

negative components

Question 3

What components (positive or negative)are more abundant in cells?

Answer 3

negative

Question 4

Using a dye that is positively charge will the cell pick or up repel the dye?

Answer 4

the cell will pick up the color

Question 5

Dyes that are negatively charged will be attracted to what components of the cell?

Answer 5

the positive components of the cell

Question 6

Most negatively charged dyes are (repelled/picked up) by the cell

Answer 6

repelled

Question 7

After the negative dying, what will cell appear as?

Answer 7

clear, with a dark background

Question 8

Simple stain definition:

Answer 8

a procedure that uses one positively charged dye t give cells color

Question 9

3 examples of a simple stain:

Answer 9

methylene blue, crystal violet, safranin and manevals

Question 10

Negative stain def:

Answer 10

a procedure that uses a negatively charged dye to stain the background while the cells remain virtually colorless

Question 11

3 examples of negative stain:

Answer 11

Eosin, india ink, nigrosin and congo red

Question 12

Differential stain def:

Answer 12

use two or more dyes to reveal differences between cells , or their parts. Most differential stains include a primary stain, counterstain, and a decolorizer