Exercise 13 -The streak plate technique

Question 1

What do you separate when using the streak plate technique?

Answer 1

• mixed cultures into pure culture

Question 2

Why is getting a pure culture important?

Answer 2

for species identification, antibiotic susceptibility testing and other necessary tests

Question 3

What type of streak plate did we use for ours?

Answer 3

the 4 quadrant method

Question 4

What is a mixed culture:

Answer 4

contains more than one species of microoraganisms

Question 5

What is a pure culture? list one example

Answer 5

contains only one species of microorgansims. A colony is an example?

Question 6

What type of media did we use in the streak plate experiment?

Answer 6

a BHI - brain heart infusion

Question 7

What quadrant should represent the best colony separation?

Answer 7

the 4 quadrant

Question 8

What’s the purpose of flame sterilizing the inoculating loop in between quadrants?

Answer 8

to make sure you don’t pick up too much bacteria into the next quadrant. Flaming it ensures you start with a clean lop into the next quadrant

Question 9

Why might there be an absence of growth in quadrants II-IV?

Answer 9

not waiting for the inoculating loop to cool can kill the organisms, not allowing it to grow.

Question 10

If you want a mixed culture from your body where is some places you could find it?

Answer 10

mouth, nose, feces, sputum, saliva, sweat