Intro to Metabolism (3) Flashcards
Metabolism
Sum total of all biochemical reactions that take place in an organism. / Sum of all energy reactions that occur in an organism.
Anabolic processes
Synthetic/Constructive biochemical reactions/build
Catabolic processes
degrative biochemical reactions/ breakdown
Catalysis
reaction aided by an enzyme to make it go faster
Molecules have ____ energy in their bonds
potential
Heat and light are a form of ___ energy because of the movement of molecules and photons
kinetic
1st law of thermodynamis:
total energy of the universe is constant
2nd law of thermodynamics:
energy can be transferred, but not destroyed, which leads to increased entropy
Exergonic reactions:
release of free energy (-∆G) / releases energy
Endergonic reactions:
use up energy / absorption of free energy (-∆G)
Spontaneous reactions are favored by: (4 factors)
- Decrease in enthalpy (-∆H)
- Increase in entropy (+∆S)
- Decrease in free energy (-∆G) and
- Increase in temperature (T)
Respiration causes free energy ___ (increase/decrease)
decrease
Photosynthesis causes free energy ____ (increase/decrease)
increase
Respiration is ____ (exergonic/endergonic)
exergonic
Photosynthesis is ____ (exergonic/endergonic)
endergonic
Respiration is ____ (endothermic/exothermic)
exothermic
Photosynthesis is ____ (endothermic/exothermic)
endothermic
Respiration is ____ (anabolic/catabolic)
anabolic
Photosynthesis is ____ (anabolic/catabolic)
catabolic
ATP hydrolysis is ____ (endergonic/exergonic)
exergonic
Phosphorylation
Adding a OPO₃⁻ group to a molecule or protein
Kinases
Name of enzymes catalyzing phosphorylation reactions
ATP synthesis is an (exergonic/endergonic) process
endergonic
ATP synthesis occurs in the ___, ___, and ___
cytoplasm, mitochondria, chloroplast
Functional groups on the side chains of enzymes that lower free energy of activation of reactions are ____ (four)
-SH, -OH, -NH₂, -COO⁻
Active site
site where enzyme and its substrate interact
Factors that can affect enzyme activity ___, ___, ___, and ___
temperature, pH, salt, substrate concentration
Vₘₐₓ achieved when
all enzyme active sites are filled with substrate
Kₘ is the ___ (short def)
rate of reaction
Kₘ is affected by
substrate concentration, pH, temperature, and ionic strength
Kₘ ___ (long def)
indicates the substrate concentration at which half the enzyme active sites are filled with substrates
Competitive Inhibitors effect on Vmax and Km is ____.
Vmax remains unchanged, Km is increased
Non-competitive Inhibitors effect on Vmax and Km is ____.
Vmax decreases, Km stays the same
catalytic subunits
binding to the substrate
regulatory subunits
binding to activator or inhibitor
Cooperativity
the binding of the activator that result in cooperative/positive/benefitial changes to the 3-D shape of the entire enzyme