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Histology 2ndYR 1st Sem Prelims > Intro To Histo > Flashcards

Intro To Histo Flashcards

1
Q

Group of cells with interrelated functions

A

Tissue

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2
Q

Extracellular matrix composition

A

Fibers & Ground substance

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3
Q

-the study of tissues, their functions, and their arrangement to constitute an organ
-a.k.a Microscopic Anatomy

A

Histology

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4
Q

Enumerate the 8 Tissue processing steps

A
  1. Fixation
  2. Decalcification
  3. Dehydration
  4. Clearing
  5. Embedding
  6. Cutting & Sectioning
  7. Staining
  8. Mounting
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5
Q

Commonly used reagent for fixation

A

Formalin

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6
Q

Step to preserve the organ and terminate cell metabolism

A

Fixation

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7
Q

Step to soften samples

A

Decalcification

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8
Q

Commonly used reagent in decalcification

A

Nitric acid

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9
Q

Step that is done by successively bathing the specimen in mixture of ethanol and water from 70% to 100%

Removes water out of tissue

A

Dehydartion

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10
Q

Commonly used reagent in dehydration

A

Ethanol

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11
Q

Step for Removal of dehydrating agent by immersing the specimen in the solvent

A

Clearing

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12
Q

Commonly used reagent for Clearing

A

Xylene & Toluene

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13
Q

Step where the tissue is placed in a melting paraffin and where clearing reagent is evaporated

A

Embedding

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14
Q

Optimal temperature for paraffin wax to melt

A

52 - 60 degrees Celcius

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15
Q

-Step to trim appropriately sized blocks
-Removal of excess paraffin

A

Cutting & Sectioning

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16
Q

Tool used for cutting & sectioning

A

Microtome

17
Q

Deparaffinization & Rehydration

A

Xylene - 5 mins
Xylene - 5 mins
Absolute Ethanol - 3 mins
Absolute Ethanol - 3 mins
95% Ethanol - 3 mins
95% Ethanol - 3 mins
Distilled water - 30s to 1 min

18
Q

Step of application of color to the tissue to highlight structures

A

Staining

19
Q

Commonly used reagent in Staining

A

Hematoxylin
Eosin

20
Q

Stain for DNA

A

Feulgen reaction

21
Q

Stain for carbohydrates

A

Periodic acid schiff

22
Q

Stain for lipids

A

Sudan black

23
Q

Stain for reticular fibers

A

Silver stain

24
Q

Average tissue processing time in Ph Laboratories

A

48 to 72hrs

25
Q

Step in placing cut sections on a slide with mounting media

A

Mounting

26
Q

For preserving specimen, prevent washing of stain, prevent contamination, and provide support

A

Mounting Media

27
Q

Types of mounting media

A

Glycerin and Resins

28
Q

Reagent used for Frozen Sections

A

Compressed CO2

29
Q

Tool for frozen sectioning

A

Cryostat

Histology 2ndYR 1st Sem Prelims (7 decks)

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