INTRO TO HEMOCYTOMETER Flashcards

1
Q

It is an instrument used to count the blood cells

A

Hemocytometer

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2
Q

It is the name given to a thick glass slide. In the centre of the slide, there is an H- shaped groove

A

Neubauer’s slide

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3
Q

What is the depth of the scale of hemocytometer

A

0.1 mm

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4
Q

Neubauer’s slide with a cover slip over it, is called a

A

Neubauer’s chamber.

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5
Q

What is the measurement of each scale

A

3 mm wide and 3 mm long

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6
Q

What is the measurement of each square

A

1 mm wide and 1 mm long

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7
Q

What is the measurement of the smaller square used for WBC counting?

A

0.25

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8
Q

total small squares

A

64 squares

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9
Q

The central square is subdivided into

A

twenty-five smaller squares

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10
Q

smaller squares is further subdivided into

A

sixteen smallest squares

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11
Q

How many smallest squares are there in central square?

A

80 squares

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12
Q

We are counting platelets in

A

in all the small squares of the
central square

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13
Q

What is the measurement of RBC small square?

A

0.2

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14
Q

What is the measurement of RBC smallest square?

A

0.5

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15
Q

The outer line is the boundary of
the square.

A

double line

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16
Q

The middle line is the boundary of
the square.

A

triple line

17
Q

What are we going to do to avoid double counting?

A

Do not count cells touching bottom line and right line

18
Q

has graduations up to
mark 101

A

RBC pipette

19
Q

It has graduations up to
mark 11

A

WBC pipette

20
Q

Size of lumen is
smaller

A

RBC pipette

21
Q

Size of bulb is smaller

A

WBC pipette

22
Q

Size of bulb is larger

A

RBC pipette

23
Q

Size of lumen is larger

A

WBC pipette

24
Q

What is the dilution factor for RBC counting

A

200

25
Q

What is the dilution factor for WBC counting

A

20

26
Q

What is the dilution factor for platelet counting

A

100