INTRO TO HEMOCYTOMETER Flashcards
It is an instrument used to count the blood cells
Hemocytometer
It is the name given to a thick glass slide. In the centre of the slide, there is an H- shaped groove
Neubauer’s slide
What is the depth of the scale of hemocytometer
0.1 mm
Neubauer’s slide with a cover slip over it, is called a
Neubauer’s chamber.
What is the measurement of each scale
3 mm wide and 3 mm long
What is the measurement of each square
1 mm wide and 1 mm long
What is the measurement of the smaller square used for WBC counting?
0.25
total small squares
64 squares
The central square is subdivided into
twenty-five smaller squares
smaller squares is further subdivided into
sixteen smallest squares
How many smallest squares are there in central square?
80 squares
We are counting platelets in
in all the small squares of the
central square
What is the measurement of RBC small square?
0.2
What is the measurement of RBC smallest square?
0.5
The outer line is the boundary of
the square.
double line
The middle line is the boundary of
the square.
triple line
What are we going to do to avoid double counting?
Do not count cells touching bottom line and right line
has graduations up to
mark 101
RBC pipette
It has graduations up to
mark 11
WBC pipette
Size of lumen is
smaller
RBC pipette
Size of bulb is smaller
WBC pipette
Size of bulb is larger
RBC pipette
Size of lumen is larger
WBC pipette
What is the dilution factor for RBC counting
200
What is the dilution factor for WBC counting
20
What is the dilution factor for platelet counting
100
