HEMOGLOBIN DETERMINATION (ACID HEMATIN & CYANMETHEMOGLOBIN) Flashcards
It is the red iron bearing protein contained within the erythrocytes in normal blood
Hemoglobin
What is the function of hemoglobin?
Carry oxygen & carbon dioxide to facilitate gas exchange
Hemoglobin is composed of
- heme molecules
- globin chains
Represents colored component
Heme
It is a complex protein
Globin
What is the molecular weight of one Hb molecule?
64,458 daltons
What are the 3 factors that affected hemoglobin?
- age
- sex
- altitude of the locality (the higher the place, the higher your hemoglobin become)
Usually used in blood bank
Copper sulfate or specific gravity method
What is the reagent of acid hematin?
0.1 normal hypochloric acid
Reagent of alkali hematin
Sodium hydroxide
Best metgod for measuring plasma hemoglobin
Oxyhemoglobin method
Reagent for cyanmethemoglobin
Drabkin’s reagent + 20 uL blood
Presence of free hemoglobin in the plasma confined with the RBCs the hemoglobin is release into the plasma
Hemoglobinemia
Decrease in anemia
Oligochromia
Hemoglobin Reference ranges for adult male
13.0-17.0 gm/dl
Hemoglobin Reference ranges for adult female (non-pregnant)
12.0-15.0 gm/dl
Hemoglobin Reference ranges for adult female (pregnant)
11.0-14.0
Critical values interpretation for < 7 gm/dl
Severe anemia
Critical values interpretation for 20 gm/dl
Hyperviscosity
All hemoglobin derivaties except _______ are measured with their conversion to cyanmethemoglobin
sulfhemoglobin
If the charge of iron within the
hemoglobin is converted from 2+ to 3+
that hemoglobin is already called ________
Methemoglobin
The one who convert hemoglobin to methemoglobin
Ferricyanide
Color of acid hematin
Brownish yellow color
Drabkin’s reagent consist of
- ferricyanide
- potassium cyanide
