Intro to Enzymology

Question 1

What are the leakage enzymes?

Answer 1

ALT
AST
SDH
GLDH
CK

Question 2

What are the induced enzymes?

Answer 2

ALP

GGT

Question 3

CK is _____ specific

Answer 3

muscle

Question 4

Do CNS injuries raise serum CK?

Answer 4

No!

Question 5

AST highest activity is associated with

Answer 5

skeletal and cardiac muscle

hepatocytes

Question 6

Causes of increased serum CK

Answer 6

skeletal muscle injury (necrosis, IM inj, trauma, strenous excercise)
commonly seen in “down” cows/horses
cardiac muscle injury
muscle break down (catabolism) - severly anorexic cats

Question 7

Ck kinetics

Answer 7

increases rapidly after injury
-peaks 6-12 hrs
Very short 1/2 life
-

Question 8

Would anorexia and malnourishment in a cat lead to an increased CK

Answer 8

Yes! And it will decrease significantly w/i 48 hrs of re-feeding

Question 9

Which increases faster, CK or AST? Which is more muscle specific?

Answer 9

CK is faster

CK is more muscle specific

Question 10

Can ALT increase with severe muscle damage?

Answer 10

Yep! skeletal muscle is 5% of liver ALT activity

Question 11

Myoglobin is released from _______

Answer 11

dead, dying muscle

generally severe, acute injury

Question 12

How can you distinguish between myoglobin and hemoglobin on UA?

Answer 12

anemia = hgb
increased ck = myoglobin
myoglobin = clear serum
myoglobin does not percipitate w/ ammonium sulfate