Intro to Enzymology Flashcards
What are the leakage enzymes?
ALT AST SDH GLDH CK
What are the induced enzymes?
ALP
GGT
CK is _____ specific
muscle
Do CNS injuries raise serum CK?
No!
AST highest activity is associated with
skeletal and cardiac muscle
hepatocytes
Causes of increased serum CK
skeletal muscle injury (necrosis, IM inj, trauma, strenous excercise)
commonly seen in “down” cows/horses
cardiac muscle injury
muscle break down (catabolism) - severly anorexic cats
Ck kinetics
increases rapidly after injury
-peaks 6-12 hrs
Very short 1/2 life
-
Would anorexia and malnourishment in a cat lead to an increased CK
Yes! And it will decrease significantly w/i 48 hrs of re-feeding
Which increases faster, CK or AST? Which is more muscle specific?
CK is faster
CK is more muscle specific
Can ALT increase with severe muscle damage?
Yep! skeletal muscle is 5% of liver ALT activity
Myoglobin is released from _______
dead, dying muscle
generally severe, acute injury
How can you distinguish between myoglobin and hemoglobin on UA?
anemia = hgb
increased ck = myoglobin
myoglobin = clear serum
myoglobin does not percipitate w/ ammonium sulfate