Intracellular Signalling - Protein Kinases Flashcards
What are the 3 main groups of protein kinases?
- Serine/Threonine kinases
- Tyrosine kinases
- Dual-specificity kinases
What is function of protein kinases?
Enzymes that facilitate the transfer of a phosphate group from ATP to a specific amino acid reside (Set, Thr or Tyr) on a specific protein
What is result of phosphorylation of a protein?
Changes the function of the protein (may activate or inhibit)
Where is the phosphate group from ATP added?
Onto the hydroxyl group (OH) on the side group of the amino acids
What do serine/threonine kinases do?
Phosphorylate Ser and/or Thr residues
PKA, PKC, PKG
What do tyrosine kinases do?
Phosphorylate only Tyr residues
Receptor TKs (insulin receptor) Non-receptor TKs (Src)
What do dual-specificity kinases do?
Phosphorylate Ser/Thr and Tyr residues
MAP kinase kinases
What are phosphatases?
Remove phosphate groups from amino acid residues to oppose the effects of kinases
What are the 2 major groups of phosphatases?
- Ser/Thr-directed phosphoprotein phosphatases (PPPs)
2. Tyr-directed phosphotyrosine phosphatases (PTPs)
Why are there not as many different phosphatases as kinases?
Due to the broad specificity of phosphatases
How can kinases modulate protein function?
- Phosphorylation of a protein leading to a conformational change that directly alters the function of that particular protein
- Phosphorylation of a transcription factor that alters gene transcription and protein expression levels
What occurs during MAP kinase cascades?
- An MAP kinase kinase kinase (MAPKKK) phosphorylates and activates an MAP kinase kinase (MAPKK)
- MAPKK phosphorylates and activates an MAP kinase (MAPK)
- MAPK phosphorylates a downstream substrate (a phosphoprotein or eve another kinase)
What are the 3 main MAP kinase cascades?
- ERK pathway
- p38 pathway
- JRK pathway
What is the ERK pathway activated by?
Growth factors and GPCR ligands
What are the p38 and JRK pathways activated by?
Stresses and inflammatory cytokines
p38 and JRK sometimes known as the stress-activated protein kinases (SAP kinases)