Intermediate Filaments Flashcards

1
Q

IF general structure

A

8 tetramers in 1 ropelike filament, many lateral contacts, subunit assembly doesn’t require ATP or GTP

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2
Q

IF Organisation

A

Nuc to Cell membrane across cytoplasm linking cells together and to ECM. No polarity so no motor function.

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3
Q

IF location and role

A

Role: tensile strength, stretch resistence
Anchoring junctions (desmo and hemides)
Cytoplasmic: Keratins (epi) Vimentin and Vim related (CT, M cells, neuroglia) Neurofilaments
Nuclear: Nuc lamina

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4
Q

Describe Keratin IFs

A

If defective allows disconnect of epi layer from basal lamina

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5
Q

Desmin: M IF

A

Mechanical stress, protective network around each myofibril at Z-disk

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6
Q

GFAP

A

Astrocyte Mechanical strength

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7
Q

Lamins

A

Inner surface of nuc envelope for struc stability of nucleus protecting DNA, anchor for chroms, TFs, nuclear pores

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8
Q

Neurofilaments

A

Axons, NF crosslinking long axon.
NF: tensile str
MT: transport

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9
Q

Role of IFs in Detection of Cancer Origin

A

Tumor cells lose normal appearance but can tell origin by IF expression. Fluorescent antibodies used to determine origin = epi, mesenchymal, neuronal tissue
Metastasis detection

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10
Q

Describe Bullosa simplex

A

Rupture of Keratin IFs connected to Basal Lamina. AD, Keratin 5/14, blisters on BM, fragile skin - minor friction/trauma = blisters

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11
Q

Describe Hyperkeratosis

A

Mut in keratin 1/10, supra basilar differentiation of keratinocytes
Defect weakens structural stability of keratinocytes leading to easy blisters
Chronic wounds = hyperproliferation = thick skin

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12
Q

Describe Plantopalmer Keratoderma

A

K9 defect = only in thick skin

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