Intercellular Communication and Principal of Endocrinology Flashcards
Communication of cells with each other is essential for..
- coordinating their diverse activities
- maintain homeostasis
- growth of cell
- development of the body as a whole
Types of cell communication
intracellular and intercellular
Intracellular control of cellular function
communication within cells of the body e.g. synthesis of NTs needs communication between RER,SER, ribosome,etc.
Intercellular (extra-) control of cellular function
communication between the cells of the body e.g. release of ACTH during stress causes release of cortisol
Types of intercellular (extra-) communication
direct and indirect
Direct intercellular
gap junction
- cells are in close vicinity
Indirect intercellular
involve 4 types of chemical messengers: paracrine, NT secretion, hormonal secretion, neurohormone secretion
- cells are far apart
- involve ECF
Direct intercellular communication: gap junctions
- intimate way of communication
- achieved through “signalling molecules” on the surface of a cell
- possesses identifying ‘markers’ on cell membranes
- does not involve ECF
- e.g. phagocytes recognize and destroy bacteria and invaders
- e.g. antibodies “marked” bacteria as foreigners to destroy them
Paracrine
- local chemical messenger exerts their effects on neighbouring cells in an immediate environment
- chemical messengers use simple diffusion and act at a short distance
- do not gain entry into the blood
- e.g. histamine release causes vasodilation of the neighbouring blood vessel
Neurotransmitters
- short-range chemical messengers
- diffuse across narrow space to act locally on adjoining target cell (another neuron, muscle or ligand)
Hormones
- long-range chemical messengers
- secreted into blood by endocrine glands in response to an appropriate signal
- exert effect on target cells some distance away from release site
- e.g. GH only binds to muscles and bones in the body
Neurohormones
- hormones are released into the blood by neurosecretory neurons
- distributed through the blood to distant target cells e.g. ADH (vasopressin- released when suffering dehydration) and oxytocin (helps bonding in newborns and mom)
Signal transduction
- the combination of extracellular chemical messenger with its membrane receptors triggers a sequence of intracellular events for particular cellular activities (i.e. transport, secretion, metabolism and contraction)
- converting one chemical signal to another using energy
Chemical messenger’s action on target cell
endocrine cells –> first (extracellular) chemical messenger –> blood –> target cell –> receptors –> respond by closing/opening specific channels in the membrane and releasing second messengers like cAMP, Ca2+-PIP2 and cGMP
First messenger
- extracellular chemical messenger binds to receptors
- open/close the specific channels to regulate the movement of a particular ion in and out of the cell OR transfer extracellular chemical messenger to the second messenger
Second messenger
triggers a preprogrammed series of the biochemical events within the cell (located inside the target tissue)
Mechanism of action of hydrophilic hormones via activation of the cyclic AMP second-messenger system
- binding of extra-cellular messenger to receptor activates a G protein, the alpha subunit of which shuttles to and activates adenylyl cyclase
- adenylyl cyclase converts ATP to cAMP
- cAMP activates protein kinase A
- protein kinase A phosphorylates inactive target protein, activating it
- active target protein brings about desired response
Functions of cAMP
- modification of heart rate
- formation of female sex hormones in the ovaries
- breakdown of stored glucose in the liver
- reception of a sweet taste by a taste bud
- conservation of water during urine formation
Activation of the calcium second-messenger system by an extracellular messenger
- binding of extracellular messenger to receptor activates a G protein, the alpha subunit of which shuttles to and activates phospholipase C
- Phospholipase C converts PIP2 to IP3 and DAG
3a. IP3 mobilizes intracellular Ca2+
3b. DAG activates protein kinase C
4a. Ca2+ activates calmodulin
4b. Protein kinase C phosphorylates inactive target protein, activating it
5a. Ca2+- calmodulin complex activates Ca2+- calmodulin-dependent protein kinase (CaM kinase)
5b. active target protein brings about desired response
6a. CaM kinase phosphorylates inactive target protein, activating it
7a. active target protein brings about desired response
cGMP (cyclic guanosine monophosphate)
in a few cells, cyclic GMP serves as a second messenger in a system analogous to the cAMP system e.g. the signal transduction pathway involved in vision
The functions of the endocrine glands (hormones)
- regulate metabolism, water and electrolyte balance by acting on the kidneys e.g. aldosterone
- induce adaptive changes to help the body cope with stressful situations e.g. cortisol
- promote growth and development e.g. GH
- control reproduction e.g. FSH and LH
- regulate blood cell production e.g. erythropoietin
- endocrine and ANS control and regulate both circulation, digestion and absorption of food in GI e.g. gastrin, CCK
the plasma concentration of free, biologically active hormone, which can interact with its target cells to produce a physiological response, depends on…
- the hormone’s rate of secretion by the endocrine gland
- its rate of metabolic activation
- its extent of binding to plasma proteins (lipophilic hormones)
- its rate of metabolic inactivation and excretion
POMC (pre-pro melanocortin) when cleaved produces active hormones:
__, ___ and ___ which target the ___, ___ and ___ respectively, and that will cause the release of __, __ ___, __ ___
POMC (pre-pro melanocortin) when cleaved produces active hormones:
ACTH, MSH and beta-endorphin which target the adrenal gland, melanocytes and analgesic respectively, and that will cause the release of cortisol, skin color, pain relief
Steroid hormones
- includes hormones secreted by the adrenal cortex, gonads and placenta
- are derivatives of cholesterol
- these hormones do not store in cells once formed and are released in blood immediately via lipid soluble plasma membrane
- these hormones undergo interconversion in blood or in other organs e.g. progesterone becomes cortisol
Amines/catecholamines
- derived from the amino acid tyrosine
- include the hormones secreted by the thyroid and adrenal medulla
- enzymes synthesizing these hormones are not located in cell organelles
- both amines are stored in the cell until their secretion
Solubility: peptides
hydrophilic
Structure: peptides
chains of specific amino acids
Synthesis: peptides
in rough endoplasmic reticulum, packaged in Golgi complex
Storage: peptides
large amounts in secretory granules
Secretion: peptides
exocytosis of granules
Transport in blood: peptides
as free hormone
Receptor site: peptides
surface of target cell
Mechanism of action: peptides
channel changes or activation of second-messenger system to alter activity of target proteins that produce the effect
Hormones of this type: peptides
majority of hormones
Solubility: catecholamines
hydrophilic
Structure: catecholamines
tyrosine derivative
Synthesis: catecholamines
in cytosol
Storage: catecholamines
in chromaffin granules
Secretion: catecholamines
exocytosis of granules
Transport in blood: catecholamines
half bound to plasma proteins
Receptor site: catecholamines
surface of target cell
Mechanism of action: catecholamines
Activation of second-messenger system to alter activity of target proteins that produce the effect
Hormones of this type: catecholamines
only hormones from the adrenal medulla
Solubility: thyroid hormone
lipophilic
Structure: thyroid hormone
iodinate tyrosine derivative
Synthesis: thyroid hormone
in colloid, an inland extracellular site
Storage: thyroid hormone
in colloid
Secretion: thyroid hormone
endocytosis of colloid
Transport in blood: thyroid hormone
mostly bound to plasma proteins
Receptor site: thyroid hormone
inside target cell
Mechanism of action: thyroid hormone
activation of specific genes to make new proteins that produce the effect
Hormones of this type: thyroid hormone
only hormones from the thyroid follicular cells
Hormones can influence the activity of another hormone at a given target cell in 3 ways…
- permissiveness
- synergism
- antagonism
Permissiveness
- one hormone must be present in adequate amounts for the full exertion of another hormone’s effect
- one hormone enhances the responsiveness of another hormone to the target gland
- e.g. thyroid hormone increases receptor for epinephrine’s target cells (cardiac muscle cells)