Instrumentation

Question 1

Measure the Hydrogen Ion concentration and the pH of an unknown solutopm

Answer 1

pH Meter

Question 2

Designate the intensity or degree of acidity

Answer 2

pH meter

Question 3

equipped with a glass electrode or reference electrode

Answer 3

pH meter

Question 4

What does most MODERN PH METERS have?

Answer 4

Temperature Probe

Question 5

Allows automatic temperature corrections since pH may vary relative to the temperature

Answer 5

Temperature probe

Question 6

Formula for Hydroxide ions

Answer 6

• log [OH]

Question 7

Formula for Hydrogen ions

Answer 7

• log [H]

Question 8

formula for computing the ph and pOH

Answer 8

pH + pOH = 14

Question 9

Consist of thin, pH sensitive glass membrane, the inside which contains the solution with constant concentration of hydrogen ions

Answer 9

Glass Electrode

Question 10

what is the most commonly used reference electrode?

Answer 10

CALOMEL ELECTRODE

Question 11

Incorporates a salt bridge filled with saturated potassium chloride solution

Answer 11

Calomel Electrode

Question 11

What is the used calibration buffers if the pH solution tested falls between 7.0 to 10.0

Answer 11

use pH 7 and pH 10 calibration buffers for the two point calibration

Question 12

Method to measure how much chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solutions

Answer 12

Spectrophotometry

Question 12

What is the used calibration buffers if the pH solution tested falls between 4.0 to 7.00

Answer 12

use pH 4 and pH 7 calibration buffers for the two point calibration

Question 13

Each compound absorbs or transmit light over a certain range of wavelength

Answer 13

Basic principle

Question 14

One of the most useful methods of quantitative analysis

Answer 14

Spectrophotometry

Question 15

what is the used instrument for spectrophotometry

Answer 15

Spectrophotometer

Question 16

It is a spectrophotometer that makes the sample absorb light in the visible and near ultraviolet region of the electromagnetic spectrum

Answer 16

Ultraviolet-visible spectrophotometer

Question 17

Are used to measure the amount of light transmitted through a sample at given wave length.

Answer 17

Colometric Assays

Question 18

What are the wavelength of light source of UV-Visible Spectrophotometer

Answer 18

Ultraviolet Range
Visible Range
IR Spectrophotometer
Infrared Range

Question 19

What is the range of Ultraviolet Range

Answer 19

185-400 nm

Question 20

What is the range of visible range?

Answer 20

400-700 nm

Question 21

What is the range of Infrared range?

Answer 21

700-1500 n,

Question 22

Enumerate the essential parts of UV-Vis Spectrophotometer

Answer 22

White light source
Monochromator
Sample compartment
Detector
Readout Device
Tungsten Lamp
Deuterium Lamp

Question 23

Disperse light into its components wavelengths

Answer 23

Monochromator

Question 24

Holds the transparent tube or cell containing the sample

Answer 24

Sample Compartment

Question 25

Measure the amount of light transmitted

Answer 25

Detector

Question 26

Display the reading

Answer 26

Readout Device

Question 27

Used as a light source in the Visible Region

Answer 27

Tungsten Lamp

Question 28

Used in ultraviolet light

Answer 28

Deuterium Lamp

Question 29

What are the 3 types of Cubett

Answer 29

Glass Plastic Fused Quartz

Question 30

What is range of glass and plastic cubett

Answer 30

380-780 nm

Question 31

What is the range of Fused quartz

Answer 31

less than 380 nm

Question 32

The amount of monochromatic light absorbed by a sample is determined by comparing the intensities of incident light (Io) and the transmitted light (I)

Answer 32

Transmittance

Question 33

The ratio of the intensity of transmitted light to the incident light is called

Answer 33

Transmittance (T)

Question 34

Formula of Transmittance

Answer 34

T = I/Io

Question 35

Formula for Percent Transmittance

Answer 35

%T = T x 100

Question 36

is the amount of light absorbed by a sample

Answer 36

Absorbance

Question 37

Formula for absorbance

Answer 37

A = -log T or A = -log 10 (1/T) or A = 2 -log 10% T

Question 38

related to transmittance in a logarithmic manner

Answer 38

Absorbance

Question 39

is the determination of the concentration of absorbing species in the analyte solution

Answer 39

Beer Lambert's Law

Question 40

States that the intensity of transmitted light decreases the exponentially as any one of these factor decreases

Answer 40

Beer's Law

Question 41

Absorbance is directly related to the concentration if the measurement are made at a fixed wavelength in a cell of constant path which is usually 1 cm

Answer 41

Beer's LAw

Question 42

Formula for Beer's Law

Answer 42

A = εbc

Question 43

measured absorbance

Answer 43

A

Question 44

Molar extinction coefficient

Answer 44

ε

Question 45

concentration in molarity

Answer 45

c

Question 46

Path length is usually 1 cm

Answer 46

I

Question 47

A measure of how strongly a solution absorbs light in particular wavelength

Answer 47

Molar Extinction Coefficient