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Biochem LAB > Instrumentation > Flashcards

Instrumentation Flashcards

1
Q

Measure the Hydrogen Ion concentration and the pH of an unknown solutopm

A

pH Meter

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2
Q

Designate the intensity or degree of acidity

A

pH meter

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3
Q

equipped with a glass electrode or reference electrode

A

pH meter

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4
Q

What does most MODERN PH METERS have?

A

Temperature Probe

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5
Q

Allows automatic temperature corrections since pH may vary relative to the temperature

A

Temperature probe

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6
Q

Formula for Hydroxide ions

A
  • log [OH]
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7
Q

Formula for Hydrogen ions

A
  • log [H]
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8
Q

formula for computing the ph and pOH

A

pH + pOH = 14

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9
Q

Consist of thin, pH sensitive glass membrane, the inside which contains the solution with constant concentration of hydrogen ions

A

Glass Electrode

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10
Q

what is the most commonly used reference electrode?

A

CALOMEL ELECTRODE

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11
Q

Incorporates a salt bridge filled with saturated potassium chloride solution

A

Calomel Electrode

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11
Q

What is the used calibration buffers if the pH solution tested falls between 7.0 to 10.0

A

use pH 7 and pH 10 calibration buffers for the two point calibration

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12
Q

Method to measure how much chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solutions

A

Spectrophotometry

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12
Q

What is the used calibration buffers if the pH solution tested falls between 4.0 to 7.00

A

use pH 4 and pH 7 calibration buffers for the two point calibration

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13
Q

Each compound absorbs or transmit light over a certain range of wavelength

A

Basic principle

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14
Q

One of the most useful methods of quantitative analysis

A

Spectrophotometry

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15
Q

what is the used instrument for spectrophotometry

A

Spectrophotometer

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16
Q

It is a spectrophotometer that makes the sample absorb light in the visible and near ultraviolet region of the electromagnetic spectrum

A

Ultraviolet-visible spectrophotometer

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17
Q

Are used to measure the amount of light transmitted through a sample at given wave length.

A

Colometric Assays

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18
Q

What are the wavelength of light source of UV-Visible Spectrophotometer

A

Ultraviolet Range
Visible Range
IR Spectrophotometer
Infrared Range

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19
Q

What is the range of Ultraviolet Range

A

185-400 nm

20
Q

What is the range of visible range?

A

400-700 nm

21
Q

What is the range of Infrared range?

A

700-1500 n,

22
Q

Enumerate the essential parts of UV-Vis Spectrophotometer

A

White light source
Monochromator
Sample compartment
Detector
Readout Device
Tungsten Lamp
Deuterium Lamp

23
Q

Disperse light into its components wavelengths

A

Monochromator

24
Q

Holds the transparent tube or cell containing the sample

A

Sample Compartment

25
Q

Measure the amount of light transmitted

A

Detector

26
Q

Display the reading

A

Readout Device

27
Q

Used as a light source in the Visible Region

A

Tungsten Lamp

28
Q

Used in ultraviolet light

A

Deuterium Lamp

29
Q

What are the 3 types of Cubett

A

Glass
Plastic
Fused Quartz

30
Q

What is range of glass and plastic cubett

A

380-780 nm

31
Q

What is the range of Fused quartz

A

less than 380 nm

32
Q

The amount of monochromatic light absorbed by a sample is determined by comparing the intensities of incident light (Io) and the transmitted light (I)

A

Transmittance

33
Q

The ratio of the intensity of transmitted light to the incident light is called

A

Transmittance (T)

34
Q

Formula of Transmittance

A

T = I/Io

35
Q

Formula for Percent Transmittance

A

%T = T x 100

36
Q

is the amount of light absorbed by a sample

A

Absorbance

37
Q

Formula for absorbance

A

A = -log T
or
A = -log 10 (1/T)
or
A = 2 -log 10% T

38
Q

related to transmittance in a logarithmic manner

A

Absorbance

39
Q

is the determination of the concentration of absorbing species in the analyte solution

A

Beer Lambert’s Law

40
Q

States that the intensity of transmitted light decreases the exponentially as any one of these factor decreases

A

Beer’s Law

41
Q

Absorbance is directly related to the concentration if the measurement are made at a fixed wavelength in a cell of constant path which is usually 1 cm

A

Beer’s LAw

42
Q

Formula for Beer’s Law

A

A = εbc

43
Q

measured absorbance

A

A

44
Q

Molar extinction coefficient

A

ε

45
Q

concentration in molarity

A

c

46
Q

Path length is usually 1 cm

A

I

47
Q

A measure of how strongly a solution absorbs light in particular wavelength

A

Molar Extinction Coefficient

48
Q
A

Biochem LAB (4 decks)

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