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Biochem LAB > Enzymes > Flashcards

Enzymes Flashcards

1
Q

Catalyst for biochemical reactions, most efficient

A

Enzymes

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2
Q

Equimolar mixture of glucose and fructose are generated

A

Invert Sugar

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3
Q

Alpha-D-glucose molecule + beta D-Fructose

A

Sucrose

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4
Q

how is dissacharide linked?

A

alpha-1,4-glycosidic bond

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5
Q

Sucrose is also known as

A

Table sugar

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6
Q

Beta-Fructofuranosidase

A

Invertase

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7
Q

biosynthesized yeast strains of Saccharomyces cerevisiae or Saccharomyces carlsbergensis

A

Invertase

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8
Q

reducing agents because of free aldhehyde group or ketone group

A

Reducing sugars

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9
Q

Enumerate reducing sugars

A

Glucose
Fructose
Lactose
Maltose
Galactose

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10
Q

Enumerate non reducingsugars

A

Sucrose

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11
Q

DNS

A

3,5-Dinitrosalicylic Acid or 2-hydroxy-3,5-Dinitrobenzoic acid

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12
Q

aromatic compound that forms 3-amino 5-nitrosalicylic acid

A

DNS

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13
Q

Strongly absorbs light at 540 nm

A

DNS

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14
Q

Detects the presence of free carbon group (C=O) of redicng sugars

A

DNS Colorimetric Method

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15
Q

Oxidation of aldhehyde in glucose and ketone in fructose

A

DNS Colorimetric Method

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16
Q

point where the Enzymes is most active or most favorable pH values

A

Optimum pH

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17
Q

Optimum pH of Lipase (pancrease)

A

8.0

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18
Q

Optimum pH of Lipase (Stomach)

A

4.0 - 5.0

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19
Q

Optimum pH of Lipase (Castor Oil)

A

4.7

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20
Q

Optimum pH of Pepsin

A

1.5-1.6

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21
Q

Optimum pH of Trypsin

A

7.8 - 8.7

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22
Q

Optimum pH of Urease

A

7.0

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23
Q

Optimum pH of Invertase

24
Q

Optimum pH of Maltase

25
Optimum pH of Amylase (pancreas)
6.7-7.0
26
Optimum pH of Amylase (malt)
4.6-5.2
27
Optimum pH of Catalase
7.0
28
what temperature does enzyme rapidly denature
above 40
29
temperature of storage of Enzymes
below 5 celsius
30
Informational molecules made up of polymers of Nucleotides linked together by phosphodiester bonds
Nucleic
31
Building blocks of nucleic acids
Nucleotides
32
Nucleotides is composed of?
Nitrogen-containing heterocycle base Phosphate Pentose sugar
33
Heterocyclic Base + Pentose Sugar
Nucleoside
34
CTAB
Cetyl-trimethylammonium bromide
35
cationic detergent-facilitates
CTABmethod
36
aid in removing polyphenols
Polvinylpyrrolidone
37
Higher salt concentrations
Polysachharides are insoluble
38
Lower salt concentratons
DNA is insoluble
39
Ctab method is used mostly for purifying DNA from?
Plant Tissues
40
What temperature is your CTAB Buffer
60 Celsius
41
low starch content, allows DNA to be seen clearly
Onion
42
DNA is not solube so DNA will precipitate
Ice-cold Ethanol
43
Test for Deoxyribose
Dische test
44
What color developers between the Dische reagent and 2-deoxyribose
Blue
45
Hydrolysis of Pyro phosphates to phosphate
Test for Phosphates
46
What is formed in the test for phosphates
Bright yellow precipitate
47
Test for purine
Murexide test
48
Ammonium purpura te or Ammonium salts of purpuric acids
Murexide test
49
test for presence of uric acids
yellow residue
50
Test for xanthine
Red residue
51
What test for presence of uric acid and xanthine
Murexide test
52
Test for pyrimidines
Wheeler Johnson Test
53
Test for pyrimidine bases cytosine and uracil
Wheeler Johnson test
54
Treated with Bromine water
Green coloration
55
addition of Barium hydroxide
Purple liquid

Biochem LAB (5 decks)

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