Inmunohistoquimica Flashcards

1
Q

Basado en:

A

Reacciones antígeno-anticuerpo

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2
Q

Reacción positiva de inmunohistoquimica:

A

Por detección de linfocitos, linfoma, cancer

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3
Q

Colores de inmunohistoquimica

A

Café y azul

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4
Q

Anticuerpos:

A

Glucoproteinas de cel inmunes, en respuesta a proteinas extrañas o antigeno

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5
Q

Anticuerpos monoclonales:

A

Producidos por una sola línea celular

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6
Q

Anticuerpos policlonales:

A

Producidos por animales inmunizados

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7
Q

Metodología de inmunohistoquimica

A

Localización de antígenos diana en celulas y tejidos, usando métodos directos o indirectos

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8
Q

Inmumnofluoresencia directa

A

Anticuerpo marcado con fluorocromo, reacciona con antigeno de la muestra
Menos sensible
Suboptima

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9
Q

Inmunofluoresencia indirecta

A

Mayor sensibilidad
Técnica de capa doble
Uso de anticuerpos secundarios
Mayor intensidad
Más laborioso

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10
Q

Inmunoperoxidasa

A

Con microscopio óptico
Directo o indirecto

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11
Q

Marcador HMB-45

A

Melanomas

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12
Q

Marcador ALK

A

Linfoma de celulas grandes anaplasico

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13
Q

Marcador CD30

A

Linfoma
Linfoblastico de cel. grandes

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14
Q

Marcador CD1a

A

Núcleo y citoplasma

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15
Q

Marcador CKAE1/AE3

A

Núcleo y citoplasma
Mío tenía marca núcleos

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16
Q

Marcador p5-100

A

Núcleos

17
Q

Marcador HVP16

A

Uso en cervix

18
Q

Marcador HBsAg

A

Usado en hígado

19
Q

Marcador HPV 6/11

A

Usado en faringe