Initiation of replication

Question 1

replicator

Answer 1

DNA sequence where imitation of replication starts, includes the origin of replication (OriC) (AT-rich) where DNA is denatured into single strands to form a replication bubble

Question 2

replication fork

Answer 2

at either end of the bubble, where DNA synthesis occurs, using each separated strand as a template

Question 3

how many origin of replication does E. coli have

Answer 3

circular genomes contain one

Question 4

how many origin of replication does eukaryote have

Answer 4

linear chromosomes contain many allowing for faster replication. proceed bidirectionaly

Question 5

initiator protein

Answer 5

initiation of replication starts with binding of initiator protein which denatures oriC and then recruits DNA helicase (one fore each strand) which untwist DNA in both directions

Question 6

where does the untwisting of DNA come from

Answer 6

hydrolysis of ATP

Question 7

DNA primase fn

Answer 7

each helicase recruits a DNA primase to form a primosome. makes the necessary RNA primers (5-10 nts) needed by DNA pol III

Question 8

define primosome

Answer 8

a protein complex responsible for creating RNA primers on ssDNA during DNA replication

Question 9

replisome

Answer 9

protein complex that carries out replication of DNA. contains helicase, primase and DNA pol and creates a replication fork to duplication leading and lagging strand