INFILTRATION/ IMPRERGNATION

Question 1

Involves removal of clearing agent from tissues

Answer 1

INFILTRATION

Question 2

Purpose/s: OF IMPREGNATION/ INFIL

Answer 2

1. To remove clearing agent from tissues
2. to fill up cavities & tissue spaces for tissues
   to have firm consistency in order to facilitate
   easy cutting

Question 3

Melting point of paraffin wax commonly used

Answer 3

56°C

Question 4

Most common method that uses heat
application
● Not suited for fatty tissues

Answer 4

Paraffin Wax Infiltration

Question 5

Uses an oven
➔ Temperature range of paraffin oven when in
used: 55-60°C
➔ Approximate temperature of paraffin oven
when in used: 2-5°C higher than the wax
melting point\
➔ Requires 4 changes of wax

Answer 5

Manual

Question 6

ses an autotechnicon
➔ Requires 3-4 changes of wax

Answer 6

AUTOMATIC

Question 7

Infiltration under negative atmospheric
pressure inside the oven
➔ Tissue processing time is reduced
➔ Facilitates removal of transition solvents
➔ Prolongs life of wax by reducing solvent
contamination

Answer 7

Vacuum

Question 8

Substitutes for Paraffin Wax

Answer 8

Paraplast
Bioloid
Carbowax
Ester Wax

Question 9

Method Recommended for specimens with
large and hollow cavities that tends to collapse
● Disadvantage: Process is slow

Answer 9

Celloidin/Collodion Infiltration

Question 10

Celloidin infiltration method recommended
forwhole eye specimens

Answer 10

Dry Celloidin

Question 11

Celloidin infiltration method for bones,
brain & teeth specimens
➔ Will require 70-80% alcohol to store blocks

Answer 11

Wet Celloidin

Question 12

Thin

Answer 12

2-14%
5-7 days

Question 13

Medium

Answer 13

4-6%
5-7 day

Question 14

Thick

Answer 14

8-12% (very high)
3-5 days

Question 15

Method for enzyme & histochemical studies
● Not usually done in the lab
● Recommended only if dehydration is to be
avoided

Answer 15

Gelatin Infiltration

Question 16

THICKNESS OF GELATIN

Answer 16

Will require tissues not to be more
than 2-3mm thick

Question 17

Serves to prevent the growth of
molds

Answer 17

Use of Pheno

Question 18

Under celloidin infiltration
● Soluble in equal concentration of Alcohol and EtheR

Answer 18

Nitrocellulose Method

Question 19

Plasticizers to prevent cracking tissues when
infiltrated using LVN (Low Viscosity
Nitrocellulose

Answer 19

Oleum Ricini & Castor Oil

Question 20

Can be used in high concentrations
● More preferred in both embedding and
infiltration method
● Harder tissue blocks are produced
● Allows cutting of thrinner sections

Answer 20

LVN