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HISTOPATH > INFILTRATION/ IMPRERGNATION > Flashcards

INFILTRATION/ IMPRERGNATION Flashcards

1
Q

Involves removal of clearing agent from tissues

A

INFILTRATION

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2
Q

Purpose/s: OF IMPREGNATION/ INFIL

A
  1. To remove clearing agent from tissues
  2. to fill up cavities & tissue spaces for tissues
    to have firm consistency in order to facilitate
    easy cutting
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3
Q

Melting point of paraffin wax commonly used

A

56°C

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4
Q

Most common method that uses heat
application
● Not suited for fatty tissues

A

Paraffin Wax Infiltration

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5
Q

Uses an oven
➔ Temperature range of paraffin oven when in
used: 55-60°C
➔ Approximate temperature of paraffin oven
when in used: 2-5°C higher than the wax
melting point\
➔ Requires 4 changes of wax

A

Manual

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6
Q

ses an autotechnicon
➔ Requires 3-4 changes of wax

A

AUTOMATIC

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7
Q

Infiltration under negative atmospheric
pressure inside the oven
➔ Tissue processing time is reduced
➔ Facilitates removal of transition solvents
➔ Prolongs life of wax by reducing solvent
contamination

A

Vacuum

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8
Q

Substitutes for Paraffin Wax

A

Paraplast
Bioloid
Carbowax
Ester Wax

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9
Q

Method Recommended for specimens with
large and hollow cavities that tends to collapse
● Disadvantage: Process is slow

A

Celloidin/Collodion Infiltration

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10
Q

Celloidin infiltration method recommended
forwhole eye specimens

A

Dry Celloidin

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11
Q

Celloidin infiltration method for bones,
brain & teeth specimens
➔ Will require 70-80% alcohol to store blocks

A

Wet Celloidin

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12
Q

Thin

A

2-14%
5-7 days

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13
Q

Medium

A

4-6%
5-7 day

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14
Q

Thick

A

8-12% (very high)
3-5 days

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15
Q

Method for enzyme & histochemical studies
● Not usually done in the lab
● Recommended only if dehydration is to be
avoided

A

Gelatin Infiltration

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16
Q

THICKNESS OF GELATIN

A

Will require tissues not to be more
than 2-3mm thick

17
Q

Serves to prevent the growth of
molds

A

Use of Pheno

18
Q

Under celloidin infiltration
● Soluble in equal concentration of Alcohol and EtheR

A

Nitrocellulose Method

19
Q

Plasticizers to prevent cracking tissues when
infiltrated using LVN (Low Viscosity
Nitrocellulose

A

Oleum Ricini & Castor Oil

20
Q

Can be used in high concentrations
● More preferred in both embedding and
infiltration method
● Harder tissue blocks are produced
● Allows cutting of thrinner sections

HISTOPATH (4 decks)

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