DEHYDRATING AGENT

Question 1

Causes only minimum shrinkage
For sections and smears

Answer 1

TRIETHYL PHOSPHATE

Question 2

Combustible at 110-120°F
 Toxic by ingestion, inhalation and skin contact
 Toxic to reproductive fetal, urinary and blood systems
 Decomposes upon exposure to sunlight

Answer 2

ETHYLENE GLYCOL MONOETHYL ETHER/
CELLUSOLVE

Question 3

PURPOSE: fix and dehydrate
 Not commonly used: evaporates easily and highly
flammable
 Only used for urgent biopsies (fast acting) and not for
routine dehydration

Answer 3

ACETONE

Question 4

Can act both as dehydrating and clearing agent
 Tissues tend to ribbon poorly
 Main disadvantage: extremely dangerous/ toxic to
man

Answer 4

DIETHYLENE DIOXIDE/ DIOXANE

Question 5

Can dehydrate and clear tissues
 Toxic if ingested or inhaled
 Vapors causes nausea, dizziness and headache
 With offensive odor, room should be well ventilated
 May cause conjunctival irritation

Answer 5

TETRAHYDROFURAN/ THF

Question 6

Used for plant & animal micro techniques

Answer 6

BUTYL ALCOHOL
BUTANOL

Question 7

ACTS AS TISSUE SOFTNER

Answer 7

PHENOL IN ALCOHOL

Question 8

TEMP TO HASTEN DEHYRATION

Answer 8

37