In Vitro Models Flashcards
What are advantages of in vitro models?
- Less Animals
- Real time observation
- Less Expense
- Patient Specific
- Controlled Environment
- Less Ethical Concerns
- Better First Look
- Scalable
What are in vitro models?
In the glass models - non animals
Disadvantages of in Vitro models?
- Antibiotics are needed
- Will always need to be done in animals
- Can take time
- No hormones so does not accurately reflect in vivo
- Longer to establish method
Examples of in vitro models?
- Cell line
- Individual follicles
etc
What was Eppig et al’s., study?
First mouse born from in vitro grown primordial cells.
How did Eppig et al., perform his study?
Follicles cultured for 22 days in vitro
- 8 days - ovaries grown
- 14 days - oocyte granulosa complex
Mature oocytes fertilised and placed in foster mother.
What did the Eppig et al., medium contain?
FSH or EGF
What was the results of the Eppig et al., study?
Two mice pups.
- One died shortly after.
- The second - Eggbert - was unhealthy.
What was the O’brien et al., study?
This was a continuation of the Eppig et al., experiment
What was the differences between the Eppig et al., study and the O’brien study?
The medium - O’brien used Fetuin and MEMa instead of Waymouth
What was the results of the O’brien study?
72 mice
What was kept the same from the O’brien and Eppig studies?
The protocol
What was the Cancer Treatment in vitro study?
Gonads removed from mouse embryo and cultured with chemo agents (Cisplatin, Etoposide and DMSO (Control)).
What did the cancer treatment in vitro study find?
Decreased follicle numbers and increase in unhealthy follicles
What method did the cancer treatment in vitro study use to see the follicles?
H and E staining, immunhistochemistry and histological analysis
How would you do the cancer treatment in vitro study in neonatal ovaries?
Do the same as the previous study but use neonatal ovaries instead.
