Immunophenotypes and Genetics for Hematologic Malignancies

Question 1

CD13+ and CD33+

Answer 1

myeloid cells. Commonly positive on all CML cases

Question 2

CD34+

Answer 2

Excessive Blasts.

Common in AML/normal genetics, AML and acute myelomonocytic

Question 3

CD117+

Answer 3

Blasts in acute myelomonocytic leukemia inv(16;16)

Question 4

t(15;17)

Answer 4

Acute promyelocytic leukemia. PML-RARA. Auer rod STACKS. Butterfly nuclei. CD34-

Question 5

t(18;21)

Answer 5

standard AML. ETO-AML. Auer Rods

Question 6

inv(16;16)

Answer 6

acute myelomonocytic leukemia. CBFB-MYH11

Question 7

CD13+, CD33+, and CD34-

Answer 7

APL. PML-RARA. t(15;17). Auer Rods and Butterfly nuclei. Thrombocytopenia, leukocytosis, and LOW blasts. (only AML with low blasts)

Question 8

CD14+, CD11b+

Answer 8

monocytes.

Common in inv(16;16) acute myelomonocytic leukemia. CBFB-MYH11. Mixed granulocytes/monocytes, eosinophilia in BM.

Question 9

t(8;21)

Answer 9

Acute myeloid leukemia. ETO-AML (RUNX1-RUNX1t1). Auer Rods and BLASTS

Question 10

t(12;21)

Answer 10

B-cell ALL

Question 11

t(9;22)

Answer 11

B-cell ALL

Question 12

t(15;17)

Answer 12

acute promyelocytic leukemia. Auer Rod Stacks. Butterfly Nuclei

Question 13

t(11;19)

Answer 13

B-cell ALL

Question 14

t(16;16)

Answer 14

acute myelomonocytic. CBFB-MYH