Immunology of pregnancy I & II Flashcards
A young woman had her 9th consecutive miscarriage. Her marriage broke down shortly afterwards.
But within months of finding a new partner, she conceived again and the pregnancy went without a hitch. Why did this happen?
Woman’s immune system took offence to the first choice of partner
over-reacting to the tissue carrying his genes and expelling the fetus.
The fetus is allogenic, its genes are half maternally and half paternally derived.
So it has paternal type antigens which are foreign to the mother. These may be recognised as foreign by the immune system.
Infertility, recurrent miscarriage, premature delivery and a dangerous complication of pregnancy, pre-eclampsia, may be strongly linked to immunological abnormalities.
BUT 350,000 babies are successfully born every day
Half of the fetal genome derives from the father but, unlike a mismatched organ transplant, it isn’t normally rejected.
How does this happen:
Immunological problems to solve during pregnancy
1) Fetal tissue is half foreign – so it has to be protected from rejection
2) But the mother’s immune defence must be sufficient during pregnancy to ensure survival of the mother.
3) Fetus often immunologically immature at birth – must have (relies on) maternal antibodies to ensure survival
The maternal/fetal interface is central to overcoming these problems. This interface occurs at the placenta
Development of the placenta
The fetus is attached with the umbilical cord
White outer part = myometrium
Pink inner part = decidua
You can see chorionic villi
Can see pools of maternal blood within the intervilus space
This is filled by spiral arteries coming from the maternal blood flow.
The interface at the placenta where it is attached to the uterine wall is key for where the maternal immune system is in contact with the cells from the fetus.
Maternal-fetal interface
The maternal-fetal interface is where the mother and babies cells are in direct contact
The cytotrophoblasts can differentiate along two pathways:
They can fuse to become syncytiotrophoblasts
Or they can differentiate to become extravillous trophoblasts
The Extravilous trophoblasts invade into the wall of the decidua and anchor the placenta.
(attached = anchoring villi, not attached = floating villi)
The chorionic villi are bathed in a large pool of maternal blood which is supplied from the maternal blood vessels: the spiral arteries.
Three interfaces:
1) Syncytiotrophoblast layer covering the placenta is bathed in maternal blood
2) Where the invading extravilous trophoblast come into contact with decidual immune cells
3) Where the invading Extravilous trophoblast come into contact with decidual blood vessels
It is difficult to study the immunology of pregnancy as most immune cells in the peripheral blood are easily accessible by taking a blood sample. But from where the blood is situated in the interfaces in pregnancy, it is very difficult to investigate this in an ongoing human pregnancy.
Most is know from animal studies, but the physiology of placentation is very different.
The only way to access the maternal fetal interface in human pregnancies is by studying cells isolated from first trimester surgical terminations of pregnancy,
BUT we do not known the outcome and if the pregnancy had developed, would it have been normal.
Maternal interface:
1) Syncytiotrophoblast layer covering the placenta is bathed in maternal blood
The syncytiotrophoblast layer that covers the placenta is bathed in maternal blood.
The syncytiotrophoblast is a multi-nucleated layer which arises from fused cytotrophoblasts.
It forms a barrier and performs endocrine functions as well as gas and nutrient exchange from maternal blood (via expression of transport proteins).
There are fetal blood vessels seen in the cross section where there is exchange between the mothers blood and the fetal blood vessels.
The syncytiotrophoblasts are in direct contact with the maternal blood which will contain immune cells:
Maternal interface:
2) Invasive extravillous trophoblast are in contact with decidual immune cells
The invasive Extravilous trophoblasts are in contact with the decidual immune cells.
The extravillous trophoblasts are differentiated fetal cells which invade into the maternal decidua to transform maternal spiral arteries.
Seen from the diagram (right):
The chorionic villi (at the top)
The invasive trophoblasts are invading downwards into the decidua and the myometrium.
The invasive Extravilous trophoblasts are somewhat like cancer cells in how they move and invade through tissue.
As they come through, they are heading towards the maternal spiral arteries.
As they come through the decidua they encounter a large infiltration of maternal immune cells:
NK cells, lymphocytes, macrophages.
All of these maternal immune cells could potentially recognise the trophoblast cells which are fetally derived, and therefore would express some of the paternal antigens as foreign. SO… the trophoblasts cells will have to successfully navigate their way through this large infiltration of maternal immune cells, so that they are able to reach the spiral arteries.
Maternal-fetal interface:
3) Invasive extravillous trophoblast are in contact with decidual vascular cells
Invasive extravillous trophoblasts are in contact with decidual vascular cells.
The third area where there is direct contact with the fetal trophoblast cells and the maternal blood cells is when they reach the spiral arteries.
The extravillous trophoblast are differentiated fetal cells which invade into the maternal decidua to transform maternal spiral arteries.
As the Extravilous trophoblasts invade they are heading towards the maternal spiral arteries.
In a non pregnant uterus, the spiral arteries are tightly coiled and are spiral in nature.
The spiral arteries are what supply all of the maternal blood to the intervillous space.
As the fetus and the placenta grows these demands increase, so a larger volume of blood needs to be supplied.
This is done through the remodelling of the spiral arteries so that they change from a low flow, high resistance vessel -> to a high flow, low resistance vessel.
The initial stages of this occur early in pregnancy alongside decidualization. This is known as the trophoblast independent remodelling stage.
Some of the immune cells have an important role in this process.
This may explain why the large infiltration of cells is needed.
Part of the trophoblast independent remodelling will be carried out by signals from some of the immune cells present in the decidua.
When the Extravilous trophoblast cells reach the vessel, they cause a loss of vascular smooth muscle layer and a temporary loss of the endothelial layer.
The lumen of the vessel will become much larger and will loose its contractile properties.
This is termed trophoblast dependant remodelling, and allows for a much increased blood supply to the intervillous space.
The trophoblasts start expressing markers of the endothelial cells and will replace the endothelial cells that line the spiral arteries.
As these cells are now fetal cells (lining the inside of the spiral artery), they will then be in direct contact with the maternal blood which is travelling through the spiral arteries.
Materal-fetal interface: summary
There will be contact between maternal cells and fetal cells in three areas:
syncytiotrophoblasts lining the chorionic villi will be in contact with maternal blood in the intervillous space
invasive extravillous trophoblasts will be in contact with maternal blood in the spiral arteries (After they have remodelled these vessels)
invasive extravillous trophoblasts will be in contact with infiltrated maternal immune cells in the decidua. (they will have to invade through all of the infiltrated maternal immune cells in the decidua)
How do both of these types of trophoblast evade the immune response?
First need to understand how the mother could mount an immune response:
Which immune cells are present at the maternal-fetal interface?
In the Decidua:
More than >40% decidual cells are leukocytes in early pregnancy. (large infiltration of immune cells).
Of these cells the predominant type, approximately 70% are NK cells (are a subpopulation of cytotoxic lymphocytes)
NK cells function by cell killing or by cytokine production
Approximately 20% of the immune cells are macrophages
T and B cells make up the remaining 10%
In the Intervillous space and spiral arteries:
The immune cells that are present will be the same as is circulating in the peripheral maternal blood
Maternal immune cells: Decidual natural killer cells (dNK)
The predominant type (70%) is the decidual natural killer cells.
dNK cells are different to peripheral blood (pb)NK cells
Their pattern of receptor expression is unique and they are identified by high expression of the protein CD56hiCD16lo peripheral blood NK cells have a low expression of CD56)
They have been identified as being essential to pregnancy in the mouse and they may also play a role in human decidual remodelling through the cytokines which they secrete
V.IMPT:
Note: how different dNK cells are to peripheral blood natural killer cells (pbNK).
When a NK cell is in the decidua, it is no longer a fierce killer cell, instead its role is more to produce cytokines that encourage the important events of placentation such as trophoblast invasion.
Maternal immune cells: Macrophages in pregnancy
The 2nd most predominant cell type are the macrophages (about 20% of immune cells in the decidua).
Another immune cell found in the decidua is the macrophage: makes up about 20% of immune cells in the decidua so is the second most abundant immune cell
dMac (decidual macrophages) have a different phenotype to peripheral blood monocytes
Broadly, macrophages may be characterised into two phenotypes M1 and M2:
M1: pro-inflammatory, they secrete TNF-α, IL-6
M2: anti-inflammatory, they secrete IL-10, VEGF
Decidual macrophages are more M2-like than M1
So are more anti-inflammatory in their nature.
Immunological tolerance:
How do trophoblast evade the immune response?
There were three different theories from 1953 regarding tolerance of the mothers immune system to the fetus, that mostly still stand today:
Physical separation of the maternal and fetal tissues
Antigenic immaturity of fetal tissues
That the mother is immunologically inert
Is there a physical separation of maternal and fetal tissues?
The Fetus is separated from the mother by the fetal trophoblast cells.
Therefore only the fetal trophoblast cells needs to have some tolerance to the mothers immune system
The fetal and maternal circulation is separated
Maternal cells cannot reach the fetus
HOWEVER:
In humans, IgG can cross into the fetal blood via a placental transport mechanism.
Therefore IgG directed against the fetal antigens could also be transferred
This is necessary for the fetal immunity to bacteria and viruses in the first weeks of neonatal life.