Immunoglobulin Gene Structure and Recombination

Question 1

D segments are used in what type of antibody chains?

Answer 1

heavy

Question 2

Light chains can be either _________ or __________.

Answer 2

kappa; lambda

Question 3

Steps of Recombination
Raging Salamanders Hide Signs Obliviously Cutting Ligers

Answer 3

R - RAG1/2 binds
S - single stranded nick
H - hydroxyl group liberated
S - signal end ligates
O - opening of hairpin
C - cleavage makes sites for P nuc
L - ligation of light chain V and J

Question 4

Heavy Chain ONLY Steps of Recombination
Excite No Ligers

Answer 4

E - exonuclease cleavage and loss nt
N - non-templated nt addition TdT
L - ligation of HC by NHEJ DNA ligase

Question 5

Five mechanisms which generate Ab Diversity
Multiple Purple Elephants Need Curry

Answer 5

M - multiple gene segments
P - p nucleotide addition
E - exonuclease trimming
N - non-templated addition by TdT
C - combinatorial diversity heavy to light chains

Question 6

What determines whether Ab will be secreted or membrane-bound?

Answer 6

mRNA splicing mechanisms also control whether the cell produced membrane bound or secreted IgM

Question 7

How are other classes of Ab made?

Answer 7

a mechanism called class switching

Question 8

Allelic exclusion ensures that each B cell synthesizes only ______ HC and ______ LC.

Answer 8

one; one