Immunofluorescence

Question 1

what is cytospin

Answer 1

is it a centrifugation process to separate layers of cells

Question 2

what are fluorochromes

Answer 2

dyes that have the ability to absorb short wavelength of UV and emit visible green light (fluorescence)

Question 3

what can cytokeratin markers do

Answer 3

can be used to indetify carcinoma

detect micrometastasis in lymph nodes

Question 4

what are monoclonal antibody

Answer 4

antibodies made by clonal unique white blood cells

Question 5

binding to the same epitope

Answer 5

can only bind to the part of the antigen that is recognised by the antibodies

Question 6

cytopin uses

Answer 6

produces a monolayer of cells on a glass slide

Question 7

role of methanol in cytospi preparation

Answer 7

odone for ten minutes
prevents protein denaturation
prevents dmage to epitope

Question 8

limitations of flourescent techniques

Answer 8

you need a fluorescent microscope to see
reduce photo bleaching by reducing the amount of light input
improper fixation in methanol decreases specifity