Immunoassay

Question 1

Study deals with serum/plasma and tests are based on antigen-antibody interaction

Answer 1

Serology/Immunology

Question 2

What tests form lattice?

Answer 2

Precipitation and Agglutination

Question 3

What test is being described? Antigen or antibody attached to latex particle.

Answer 3

Latex agglutination

Question 4

What label is used for IFA?

Answer 4

Fluorescin isothiocyanate

Question 5

What are the uses of IFA?

Answer 5

Autoantibodies (ANA, anti-thyroid, anti-mitochondrial), Infectious agents (Hepatitis, syphilis, etc)

Question 6

T or F. Tumor markers are used for screening.

Answer 6

False. They are used to monitor the tumor.

Question 7

What tumor marker is used for colorectal cancer?

Answer 7

CEA

Question 8

What tumor marker is used for ovarian CA?

Answer 8

CA125

Question 9

What tumor marker is used for breast CA?

Answer 9

CA15-3

Question 10

What tumor marker is used for testicular cancer?

Answer 10

Beta HCG

Question 11

What labs are needed to test for Hepatitis A?

Answer 11

IgM antiHAV. If positive, then you are in an acute infection or recovery phase. IgG not necessary

Question 12

(-) HBsAg, (+) anti-HBc, (+) anti-HBS

Answer 12

Immune due to natural infection

Question 13

(+) HBsAg, (+) anti-HBc, (-) anti-HBS, (+) IgM anti-HBc

Answer 13

Acute infection

Question 14

Elevated TSH, Low FT4

Answer 14

Primary hypothyroidism

Question 15

Elevated TSH, High FT4

Answer 15

TSH-mediated hyperthyroidism

Question 16

Low TSH, High FT4

Answer 16

Hyperthyroidism, thyrotoxicosis

Question 17

Low TSH, Low FT4

Answer 17

Central hypothyroidism