IHC Flashcards
1
Q
What is an antigen?
A
- foreign body
- initiates immune reponse
2
Q
What are antibodies?
A
- immunoglobulins
- made to neutralise antigens
3
Q
What is an Epitope
A
-binding site on anitgen for anitbody to bind to
4
Q
Polyclonal antibodies are…
A
- preparations containing antibodies against more than one epitope of an antigen
- made by injecting antigen into an animal
- easy to obtain
- tolerant to minor changes in epitope
5
Q
Monoclonal antibodies are…
A
-highly specific antibodies produced in large quantities by the clones of a single hybrid cell formed by the fusion of a B cell with a tumour cell
-mouse or rat is injected with antigen & antibody-
forming cells are isolated from the spleen
-good if large quantities needed
