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FINALS - bacte > ID OLF STREP > Flashcards

ID OLF STREP Flashcards

1
Q

gram stain of strep

A

Gram positive cocci in pairs or chains

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2
Q

cultural characteristics of strep

A

pinpoint colonies

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3
Q

cultural characteristics in SBA group A - Streptococcus pyogenes

A

small, transparent and smooth;
beta-hemolytic; when grows in sheep’s blood agar

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4
Q

cultural characteristics in SBA group B - Streptococcus agalactiae

A

grayish white mucoid colonies;
beta-hemolytic (small zone)

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5
Q

catalase test result of strep

A

negative

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6
Q

Differentiates group A from other strep groups

A

Bacitracin Susceptibility Test (Taxo A)

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7
Q

Bacitracin Susceptibility Test (Taxo A) uses what sample

A

throat swab

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8
Q

a throat swab is used in Bacitracin Susceptibility Test (Taxo A) to inoculate in SBA containing ____ and ____

A

sulfamethoxazole and a bacitracin
disk

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9
Q

CAMP TEST stands for

A

● Christie, Atkinson, Munch, and Peterson

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10
Q

CAMP test is test for?

A

Group B - S. aglactiae

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11
Q

CAMP test is test for?

A

Group B - S. agalactiae

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12
Q

positive result of CAMP test

A

arrowhead hemolysis or bowtie appearance

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13
Q

Positive result for PYR hydrolysis Test

A

cherry red/pink

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14
Q

PYR hydrolysis test is test for

A

group A and group D

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15
Q

reagent for PYR hydrolysis test

A

p-dimethyl-aminocinnamaldehyde

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16
Q

the only specie of strep that is PYR positive

A

S pyogenes

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17
Q

PYR test determines the activity of an enzyme called

A

pyroglutamyl aminopeptidase

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18
Q

Hippurate test is for?

A

Group B

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19
Q

reagent indicator of hippurate test

A

ninhydrin

20
Q

positive result for hippurate test

A

purple

21
Q

salt intolerance test percentage of salt

A

six point five percent NaCl

22
Q

The ______ test is a particularly important test used
to determine whether the gram positive cocci is a
staphylococci or a streptococci

A

catalase

23
Q

enzyne detected in hippurate test

A

hippuricase

24
Q

Hippurate hydrolysis test is used to detect the ability of
bacteria to hydrolyze substrate hippurate into ____ and ___by the action of the Hippuricase
enzyme present in the bacteria

A

glycine
and benzoic acid

25
Q

principle behind camp test

A

Certain organisms including group B streptococci
produce a diffusible extracellular hemolytic heat stable
protein known as the camp factor that acts
synergistically with a beta lysin of staphylococcus aureus
to cause enhanced lysis of red blood cells.

26
Q

principle behind hippurate test

A

Hippurate hydrolysis test is used to detect the ability of
bacteria to hydrolyze substrate hippurate into glycine
and benzoic acid by the action of the Hippuricase
enzyme present in the bacteria

27
Q

test specifically for Group D strep

A

bile esculin test

28
Q

enzyme involve in bile esculin test

A

esculinase

29
Q

positive reaction in bile esculin test

A

blackening of medium

30
Q

Principle behind bile esculin test

A

test for group D species of strep that are bile tolerant and can hydrolyzes esculin to esculetin through the use of enzyme esculinase

31
Q

percentage of salt in salt tolerance test

A

6.5% Nacl

32
Q

a further test for bile esculin test that determines if the group d specie is enterococci or non-enterococci

A

salt tolerance test (6.5% NaCl)

33
Q

negative reaction in salt tolerance means

A

a clear/transparent broth means non-enterococci

34
Q

reagent of Leucine aminopeptidase test

A

paradimethylaminocinnamaldehyde

35
Q

positive reaction of Leucine aminopeptidase

A

red color

36
Q

species positive in leucine aminopeptidase

A

viridans streptococci

37
Q

this susceptibility test in
conjunction with bacitracin is used for the presumptive
identification of beta hemolytic streptococci on blood
agar

A

SXT or sulfamethoxazole Susceptibility Test

38
Q

positive in SXT or sulfamethoxazole test

A

Resistant – Group A & B Strep

39
Q

steps in gram stain strep

A
  1. Using a sterile loop, place a loopful of saline into a labelled slide
  2. Emulsify a colony from Plate into the saline
  3. Air dry and heat fix
  4. Stain with gram stain
  5. View under microscope
40
Q

steps in catalase test strep

A
  1. Place 1 drop of hydrogen peroxide reagent in a glass slide
  2. Emulsify a colony from the plate using applicator stick
  3. Observe
    (+) bubble formation
    (-) no gas bubbles
41
Q

steps in bacitracin dic test (taxo a)

A
  1. Make a bacterial suspension (pure inoculum) by placing 3-5 colonies from plate into sterile
    distilled water. Match the turbidity with the MacFarland standard
  2. Using lawn culture method, streak the pure inoculum into a blood agar plate
  3. After drying for 10 minutes, place the 0.04 units bacitracin disc into the blood agar plate
    using sterile forceps
  4. Incubate
    (+) Zone of Inhibition (any size)
    (-) No zone of inhibition
42
Q

steps in camp test

A
  1. Inoculate beta-toxin producing S. aureus straight on the center of BAP
  2. Using bacteria from plate, inoculate a straight line at right angle to Staphylococcus streak.
    Make sure the two streak lines do not touch.
  3. Incubate at 35 C for 18 hours in a candle jar
  4. Observe
    (+) Arrow head hemolysis at the junction of the colonies
43
Q

steps in identifying colonial morphology of strep

A
  1. Using multiple interrupted streak, inoculate a BAP with bacteria from plate.
  2. Incubate for 18-24 hours
    Take note of colony morphology: color, size, hemolytic pattern
44
Q

steps in hippurate hydrolysis

A
  1. Inoculate bacteria on sodium Hippurate substrate to produce a milky suspension
  2. Incubate at 35 C for 2 hours
  3. Add 0.2 mL ninhydrin reagent and further incubate for 15 minutes
    (+) Purple color
45
Q

steps in bile esculin test

A
  1. Using multiple interrupted streak, inoculate a BEA plate with bacteria from plate.
  2. Incubate for 18-24 hours
    (+) Growth and Blackening of agar
46
Q

steps in salt tolerance test

A
  1. Inoculate loopful of bacteria to 6.5% NaCl broth
  2. Incubate at 35 C for 18 hours
    (+) turbidity

FINALS - bacte (7 decks)

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