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FINALS - bacte > ID OF BACTERIACEAE > Flashcards

ID OF BACTERIACEAE Flashcards

1
Q

Ability to ferment glucose, lactose, and sucrose and to
produce hydrogen sulfide

A

Triple Sugar Iron Agar (TSI)

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2
Q

Composition of TSI agar

A

: 1% lactose, 1% sucrose, 0.1% glucose

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3
Q

what is added in TSI to detect the production of hydrogen sulfide

A

Ferrous sulfate and sodium thiosulfate

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4
Q

pH indicator in TSI agar

A

phenol red

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5
Q

orginal color of the TSI agar

A

red

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6
Q

is a similar medium to TSI, but only
incorporates the carbohydrates glucose and lactose.
- H2S production can also be detected with this
formulation

A

Kligler’s iron agar (KIA)

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7
Q
  • Phenol red is used as the pH indicator which is
    yellow below the pH of ___. An inoculated medium is red because the pH is buffered at _____
A

6.8, 7.4.

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8
Q

glucose fermentation in TSI

A

ALK SLANT/ACID BUTT or K/A

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9
Q

IMViC stands for

A

● Indole Test
● Methyl Red Test
● Voges-Proskauer Test
● Citrate Utilization Test

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10
Q

Test the ability of the bacteria to produce the
enzyme tryptophanase and deaminate
tryptophan to indole, pyruvic acid, and
ammonia

A

INDOLE TEST

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11
Q

Test that deaminate TRYPTOPHAN

A

INDOLE TEST

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12
Q

ENZYME OF INDOLE TEST

A

tryptohanase

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13
Q

Production of indole is detected using

A

Ehrlich’s reagent or Kovac’s reagent

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14
Q

Positive reaction of the two mentioned reagent on the previous card

A

pink/red, red ring at the top

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15
Q

If glucose is metabolized by the mixed acid
fermentation pathway, stable acid end
products are produced, which results in a low
pH

A

METHYL RED TEST

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16
Q

Positive reaction of methylk red test

A

red color

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17
Q

In TSI, When sugars are fermented, acid production will occur turning the original color of the medium red into
_____

A

yellow

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18
Q

Format of writing TSI results

A

Slant/butt

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19
Q

How many hours is the TSI should be read before erroneous result override

A

18-24 hrs

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20
Q

What does red/red mean in TSI

A

No fermentation = Alkaline/alkaline

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21
Q

What does red/yellow mean in TSI

A

Glucose fermentation alkaline/acid

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22
Q

what does yellow/yellow mean in TSI

A

lactose fermentation

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23
Q

what dies blackening if the medium mean in TSI

A

Hydrogen sulfide production

24
Q

in TSI if the medium has bubbles then the organism is called ___

A

aerogenic

25
Q

among the 2 reagents in Indole test, which one is more sensitive?

A

Ehrlich’s reagent

26
Q

what is added in Methyl red test?

A

MR pH indicator

27
Q

_____ and _______
detects the end products of glucose
fermentation

A

Methyl Red and Voges-Proskauer Test

28
Q

It measure the production of acetoin

A

VOGES-PROSKAUER TEST

29
Q

VOGES-PROSKAUER TEST are measrued after addition of ____

A

alpha-naphthol followed by 40% KOH or NaOH (potassium or soddium hydroxide)

30
Q

in VOGES-PROSKAUER TEST, acetoin is oxidized into

A

diacetyl 2,3-Butanediol

31
Q

positive reaction in VOGES-PROSKAUER TEST

A

red complex

32
Q

It determines whether an organism can use
sodium citrate as a sole carbon source

A

CITRATE UTILIZATION TEST

33
Q

carbohydrates source in citrate utilization test

A

sodium citrate

34
Q

medium used in citrate utilization test

A

Simmons’ Citrate Medium
(SCA)

35
Q

nitrogen source in citrate utilization test

A

ammonium salts

36
Q

the utilization of this nitrogen salt results in the release of ___ causing a pH change

A

ammonia

37
Q

pH indicator in citrate iuutiliozation test

A

bromothymol blue

38
Q

positive reaction in citrate utilization test

A

blue

39
Q

Determines whether an organism has the ability to
reduce nitrate to nitrite

A

nitrate reduction test

40
Q

positive reaction in nitrate reduaction test

A

red , diazo red dye

41
Q

Nitrate reduction test is a test that determines
the production of an enzyme called ____

A

nitrate
reductase,

42
Q

what is added in nitrate reduction test after incubation

A

N,N-Dimethyl-α-naphthylamine and sulfanilic
acid

43
Q

Differentiates Enterobacteriaceae (oxidase-negative)
from Plesiomonas (oxidase-positive)

A

oxidase test

44
Q

reagent in oxidase test

A

tetramethyl-p-phenylenediamine dihydrochloride

45
Q

positive reaction of oxidase test

A

purple or lavender color

46
Q

enzyme in oxidase test

A

cytochrome C oxidase

47
Q

Determines whether a microorganism can hydrolyze urea

A

urease test

48
Q

enzyme in urease test

A

urease enzyme

49
Q

indicator in urease test

A

phenol red

50
Q

positive reaction in urease test

A

bright pink colorr

51
Q

It is commonly used to distinguish the
genus proteus from other enteric bacteria.

A

urease test

52
Q

positive result in gelatin hydrolysis test

A

Liquefaction

53
Q

perecent of agar in motility test medium

A

0.4%

54
Q

positive reaction in motility test

A

movement away the stab line or
hazy appearance throughout the medium

55
Q

routine medium used in motility test

A

SIM (Sulfide-Indole-Motility)
Agar

FINALS - bacte (7 decks)

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