ID OF BACTERIACEAE Flashcards
Ability to ferment glucose, lactose, and sucrose and to
produce hydrogen sulfide
Triple Sugar Iron Agar (TSI)
Composition of TSI agar
: 1% lactose, 1% sucrose, 0.1% glucose
what is added in TSI to detect the production of hydrogen sulfide
Ferrous sulfate and sodium thiosulfate
pH indicator in TSI agar
phenol red
orginal color of the TSI agar
red
is a similar medium to TSI, but only
incorporates the carbohydrates glucose and lactose.
- H2S production can also be detected with this
formulation
Kligler’s iron agar (KIA)
- Phenol red is used as the pH indicator which is
yellow below the pH of ___. An inoculated medium is red because the pH is buffered at _____
6.8, 7.4.
glucose fermentation in TSI
ALK SLANT/ACID BUTT or K/A
IMViC stands for
● Indole Test
● Methyl Red Test
● Voges-Proskauer Test
● Citrate Utilization Test
Test the ability of the bacteria to produce the
enzyme tryptophanase and deaminate
tryptophan to indole, pyruvic acid, and
ammonia
INDOLE TEST
Test that deaminate TRYPTOPHAN
INDOLE TEST
ENZYME OF INDOLE TEST
tryptohanase
Production of indole is detected using
Ehrlich’s reagent or Kovac’s reagent
Positive reaction of the two mentioned reagent on the previous card
pink/red, red ring at the top
If glucose is metabolized by the mixed acid
fermentation pathway, stable acid end
products are produced, which results in a low
pH
METHYL RED TEST
Positive reaction of methylk red test
red color
In TSI, When sugars are fermented, acid production will occur turning the original color of the medium red into
_____
yellow
Format of writing TSI results
Slant/butt
How many hours is the TSI should be read before erroneous result override
18-24 hrs
What does red/red mean in TSI
No fermentation = Alkaline/alkaline
What does red/yellow mean in TSI
Glucose fermentation alkaline/acid
what does yellow/yellow mean in TSI
lactose fermentation
what dies blackening if the medium mean in TSI
Hydrogen sulfide production
in TSI if the medium has bubbles then the organism is called ___
aerogenic
among the 2 reagents in Indole test, which one is more sensitive?
Ehrlich’s reagent
what is added in Methyl red test?
MR pH indicator
_____ and _______
detects the end products of glucose
fermentation
Methyl Red and Voges-Proskauer Test
It measure the production of acetoin
VOGES-PROSKAUER TEST
VOGES-PROSKAUER TEST are measrued after addition of ____
alpha-naphthol followed by 40% KOH or NaOH (potassium or soddium hydroxide)
in VOGES-PROSKAUER TEST, acetoin is oxidized into
diacetyl 2,3-Butanediol
positive reaction in VOGES-PROSKAUER TEST
red complex
It determines whether an organism can use
sodium citrate as a sole carbon source
CITRATE UTILIZATION TEST
carbohydrates source in citrate utilization test
sodium citrate
medium used in citrate utilization test
Simmons’ Citrate Medium
(SCA)
nitrogen source in citrate utilization test
ammonium salts
the utilization of this nitrogen salt results in the release of ___ causing a pH change
ammonia
pH indicator in citrate iuutiliozation test
bromothymol blue
positive reaction in citrate utilization test
blue
Determines whether an organism has the ability to
reduce nitrate to nitrite
nitrate reduction test
positive reaction in nitrate reduaction test
red , diazo red dye
Nitrate reduction test is a test that determines
the production of an enzyme called ____
nitrate
reductase,
what is added in nitrate reduction test after incubation
N,N-Dimethyl-α-naphthylamine and sulfanilic
acid
Differentiates Enterobacteriaceae (oxidase-negative)
from Plesiomonas (oxidase-positive)
oxidase test
reagent in oxidase test
tetramethyl-p-phenylenediamine dihydrochloride
positive reaction of oxidase test
purple or lavender color
enzyme in oxidase test
cytochrome C oxidase
Determines whether a microorganism can hydrolyze urea
urease test
enzyme in urease test
urease enzyme
indicator in urease test
phenol red
positive reaction in urease test
bright pink colorr
It is commonly used to distinguish the
genus proteus from other enteric bacteria.
urease test
positive result in gelatin hydrolysis test
Liquefaction
perecent of agar in motility test medium
0.4%
positive reaction in motility test
movement away the stab line or
hazy appearance throughout the medium
routine medium used in motility test
SIM (Sulfide-Indole-Motility)
Agar
